Design and Construction of Higher-Order Structure and Function in Proteinosome-Based Protocells

Xin Huang, Avinash J. Patil, Mei Li, Stephen Mann*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

75 Citations (Scopus)

Abstract

The design and construction of higher-order structure and function in proteinosome microcompartments enclosed by a cross-linked membrane of amphiphilic bovine serum albumin/poly(N-isopropylacrylamide) (BSA-NH2/PNIPAAm) nanoconjugates is described. Three structure/function relationships are investigated: (i) differential chemical cross-linking for the control of membrane disassembly and regulated release of encapsulated genetic polymers; (ii) enzyme-mediated hydrogel structuring of the internal microenvironment to increase mechanical robustness and generate a molecularly crowded reaction environment; and (iii) self-production of a membrane-enclosing outer hydrogel wall for generating protease-resistant forms of the protein-polymer protocells. Our results highlight the potential of integrating aspects of supramolecular and polymer chemistry into the design and construction of novel bioinspired microcompartments as a step toward small-scale materials systems based on synthetic cellularity.

Original languageEnglish
Pages (from-to)9225-9234
Number of pages10
JournalJournal of the American Chemical Society
Volume136
Issue number25
DOIs
Publication statusPublished - 25 Jun 2014

Keywords

  • BIOINORGANIC PROTOCELLS
  • ARTIFICIAL CELL
  • GENE-EXPRESSION
  • LIPID VESICLES
  • MEMBRANE
  • MODEL
  • STEP
  • COMPARTMENTALIZATION
  • MICRODROPLETS
  • PERMEABILITY

Projects

Protolife-inspired materials chemistry

Mann, S.

23/06/1422/06/17

Project: Research

Cite this