Abstract
Clustering of type II tumor necrosis factor (TNF) receptors (TNFRs) is essential for their activation, yet currently available drugs fail to activate signaling. Some strategies aim to cluster TNFR by using multivalent streptavidin or scaffolds based on dextran or graphene. However, these strategies do not allow for control of the valency or spatial organization of the ligands, and consequently control of the TNFR activation is not optimal. DNA origami nanostructures allow nanometer-precise control of the spatial organization of molecules and complexes, with defined spacing, number and valency. Here, we demonstrate the design and characterization of a DNA origami nanostructure that can be decorated with engineered single-chain TNF-related apoptosis-inducing ligand (SC-TRAIL) complexes, which show increased cell killing compared to SC-TRAIL alone on Jurkat cells. The information in this chapter can be used as a basis to decorate DNA origami nanostructures with various proteins, complexes, or other biomolecules.
| Original language | English |
|---|---|
| Title of host publication | Imaging Cell Signalling |
| Publisher | Humana Press |
| Pages | 35-53 |
| Number of pages | 19 |
| ISBN (Electronic) | 9781071638347 |
| ISBN (Print) | 9781071638330 |
| DOIs | |
| Publication status | Published - 7 May 2024 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 2800 |
| ISSN (Print) | 1064-3745 |
| ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.
Keywords
- Nanostructures/chemistry
- Humans
- Jurkat Cells
- DNA/chemistry
- TNF-Related Apoptosis-Inducing Ligand/chemistry
- Receptors, Tumor Necrosis Factor/metabolism
- Nanotechnology/methods
- Nucleic Acid Conformation