Design of a multicellular feedback control strategy in a synthetic bacterial consortium

Gianfranco Fiore; Antoni Matyjaszkiewicz; Fabio Annunziata; Claire Grierson; Nigel J. Savery; Lucia Marucci; Mario Di Bernardo

doi:10.1109/CDC.2016.7798771

Design of a multicellular feedback control strategy in a synthetic bacterial consortium

Gianfranco Fiore, Antoni Matyjaszkiewicz, Fabio Annunziata, Claire Grierson, Nigel J. Savery, Lucia Marucci, Mario Di Bernardo^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

4 Citations (Scopus)

422 Downloads (Pure)

Abstract

Living organisms employ endogenous negative feedback loops to maintain homeostasis despite environmental fluctuations. An intriguing challenge in Synthetic Biology is that of designing and implementing synthetic circuits to control host cells’ behavior, thus mimicking what natural evolution has refined and conserved. The high degree of circuit complexity required to accomplish this task, and the intrinsic modularity of classical control schemes, suggest the implementation of synthetic endogenous feedback loops across more than one cell population. The distribution of the sensing, computation and actuation functions required to achieve regulation, to different cell populations within a consortium allows to reduce the genetic engineering in a particular cell and to increase the robustness as well as the possibility of reusing the synthesized circuits. Here we propose and study, in-silico, the design of a synthetic microbial consortium implementing a feedback controller across two cell populations.

Original language	English
Title of host publication	2016 IEEE 55th Conference on Decision and Control (CDC 2016)
Subtitle of host publication	Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA
Publisher	Institute of Electrical and Electronics Engineers (IEEE)
Pages	3338-3343
Number of pages	6
ISBN (Electronic)	9781509018376
ISBN (Print)	9781509018383
DOIs	https://doi.org/10.1109/CDC.2016.7798771
Publication status	Published - Apr 2017
Event	55th IEEE Conference on Decision and Control, CDC 2016 - Las Vegas, United States Duration: 12 Dec 2016 → 14 Dec 2016

Conference

Conference	55th IEEE Conference on Decision and Control, CDC 2016
Country/Territory	United States
City	Las Vegas
Period	12/12/16 → 14/12/16

Research Groups and Themes

Bristol BioDesign Institute
Engineering Mathematics Research Group

Keywords

synthetic biology

Access to Document

10.1109/CDC.2016.7798771

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Fiore, G., Matyjaszkiewicz, A., Annunziata, F., Grierson, C., Savery, N. J., Marucci, L., & Di Bernardo, M. (2017). Design of a multicellular feedback control strategy in a synthetic bacterial consortium. In 2016 IEEE 55th Conference on Decision and Control (CDC 2016): Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA (pp. 3338-3343). Article 7798771 Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/CDC.2016.7798771

Fiore, Gianfranco ; Matyjaszkiewicz, Antoni ; Annunziata, Fabio et al. / Design of a multicellular feedback control strategy in a synthetic bacterial consortium. 2016 IEEE 55th Conference on Decision and Control (CDC 2016): Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA. Institute of Electrical and Electronics Engineers (IEEE), 2017. pp. 3338-3343

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abstract = "Living organisms employ endogenous negative feedback loops to maintain homeostasis despite environmental fluctuations. An intriguing challenge in Synthetic Biology is that of designing and implementing synthetic circuits to control host cells{\textquoteright} behavior, thus mimicking what natural evolution has refined and conserved. The high degree of circuit complexity required to accomplish this task, and the intrinsic modularity of classical control schemes, suggest the implementation of synthetic endogenous feedback loops across more than one cell population. The distribution of the sensing, computation and actuation functions required to achieve regulation, to different cell populations within a consortium allows to reduce the genetic engineering in a particular cell and to increase the robustness as well as the possibility of reusing the synthesized circuits. Here we propose and study, in-silico, the design of a synthetic microbial consortium implementing a feedback controller across two cell populations.",

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author = "Gianfranco Fiore and Antoni Matyjaszkiewicz and Fabio Annunziata and Claire Grierson and Savery, {Nigel J.} and Lucia Marucci and {Di Bernardo}, Mario",

year = "2017",

month = apr,

doi = "10.1109/CDC.2016.7798771",

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Fiore, G, Matyjaszkiewicz, A, Annunziata, F, Grierson, C , Savery, NJ , Marucci, L & Di Bernardo, M 2017, Design of a multicellular feedback control strategy in a synthetic bacterial consortium. in 2016 IEEE 55th Conference on Decision and Control (CDC 2016): Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA., 7798771, Institute of Electrical and Electronics Engineers (IEEE), pp. 3338-3343, 55th IEEE Conference on Decision and Control, CDC 2016, Las Vegas, United States, 12/12/16. https://doi.org/10.1109/CDC.2016.7798771

Design of a multicellular feedback control strategy in a synthetic bacterial consortium. / Fiore, Gianfranco; Matyjaszkiewicz, Antoni; Annunziata, Fabio et al.
2016 IEEE 55th Conference on Decision and Control (CDC 2016): Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA. Institute of Electrical and Electronics Engineers (IEEE), 2017. p. 3338-3343 7798771.

Research output: Chapter in Book/Report/Conference proceeding › Conference Contribution (Conference Proceeding)

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T1 - Design of a multicellular feedback control strategy in a synthetic bacterial consortium

AU - Fiore, Gianfranco

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AU - Grierson, Claire

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AU - Marucci, Lucia

AU - Di Bernardo, Mario

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AB - Living organisms employ endogenous negative feedback loops to maintain homeostasis despite environmental fluctuations. An intriguing challenge in Synthetic Biology is that of designing and implementing synthetic circuits to control host cells’ behavior, thus mimicking what natural evolution has refined and conserved. The high degree of circuit complexity required to accomplish this task, and the intrinsic modularity of classical control schemes, suggest the implementation of synthetic endogenous feedback loops across more than one cell population. The distribution of the sensing, computation and actuation functions required to achieve regulation, to different cell populations within a consortium allows to reduce the genetic engineering in a particular cell and to increase the robustness as well as the possibility of reusing the synthesized circuits. Here we propose and study, in-silico, the design of a synthetic microbial consortium implementing a feedback controller across two cell populations.

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Fiore G, Matyjaszkiewicz A, Annunziata F, Grierson C , Savery NJ , Marucci L et al. Design of a multicellular feedback control strategy in a synthetic bacterial consortium. In 2016 IEEE 55th Conference on Decision and Control (CDC 2016): Proceedings of a meeting held 12-14 December 2016, Las Vegas, Nevada, USA. Institute of Electrical and Electronics Engineers (IEEE). 2017. p. 3338-3343. 7798771 Epub 2016 Dec 27. doi: 10.1109/CDC.2016.7798771

Design of a multicellular feedback control strategy in a synthetic bacterial consortium

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