Designer artificial membrane binding proteins direct stem cells to the myocardium

Wenjin Xiao, Tom I P Green, Xiaowen Liang, Rosalia Cuahtecontzi Delint, Guillaume Perry, Michael S Roberts, Kris Le Vay, Catherine Back, Raimondo Ascione, Haolu Wang, Paul Race, Adam Perriman

Research output: Contribution to journalArticle (Academic Journal)

2 Citations (Scopus)
132 Downloads (Pure)

Abstract

We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a
chimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymer
surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach
significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.
Original languageEnglish
Pages (from-to)7610 - 7618
Number of pages9
JournalChemical Science
Volume10
Issue number32
DOIs
Publication statusPublished - 3 Jul 2019

Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

Keywords

  • Synthetic biology

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  • Datasets

    Chemical Science Xiao et al

    Perriman, A. W. (Contributor), Carter, B. (Creator), Zampetakis, I. (Creator), Zhang, W. (Creator), Cuahtecontzi Delint, R. (Creator), Klemperer, R. G. (Creator), Day, G. J. (Creator), Richardson, T. (Creator), Hickton, B. R. (Creator) & Coe, D. (Creator), University of Bristol, 3 Jul 2019

    Dataset

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