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Designer artificial membrane binding proteins direct stem cells to the myocardium

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Designer artificial membrane binding proteins direct stem cells to the myocardium. / Xiao, Wenjin; Green, Tom I P; Liang, Xiaowen; Cuahtecontzi Delint, Rosalia; Perry, Guillaume; Roberts, Michael S; Le Vay, Kris; Back, Catherine; Ascione, Raimondo; Wang, Haolu; Race, Paul; Perriman, Adam.

In: Chemical Science, Vol. 2019, No. 32, 03.07.2019, p. 7610 - 7618.

Research output: Contribution to journalArticle

Harvard

Xiao, W, Green, TIP, Liang, X, Cuahtecontzi Delint, R, Perry, G, Roberts, MS, Le Vay, K, Back, C, Ascione, R, Wang, H, Race, P & Perriman, A 2019, 'Designer artificial membrane binding proteins direct stem cells to the myocardium', Chemical Science, vol. 2019, no. 32, pp. 7610 - 7618. https://doi.org/10.1039/C9SC02650A

APA

Xiao, W., Green, T. I. P., Liang, X., Cuahtecontzi Delint, R., Perry, G., Roberts, M. S., ... Perriman, A. (2019). Designer artificial membrane binding proteins direct stem cells to the myocardium. Chemical Science, 2019(32), 7610 - 7618. https://doi.org/10.1039/C9SC02650A

Vancouver

Xiao W, Green TIP, Liang X, Cuahtecontzi Delint R, Perry G, Roberts MS et al. Designer artificial membrane binding proteins direct stem cells to the myocardium. Chemical Science. 2019 Jul 3;2019(32):7610 - 7618. https://doi.org/10.1039/C9SC02650A

Author

Xiao, Wenjin ; Green, Tom I P ; Liang, Xiaowen ; Cuahtecontzi Delint, Rosalia ; Perry, Guillaume ; Roberts, Michael S ; Le Vay, Kris ; Back, Catherine ; Ascione, Raimondo ; Wang, Haolu ; Race, Paul ; Perriman, Adam. / Designer artificial membrane binding proteins direct stem cells to the myocardium. In: Chemical Science. 2019 ; Vol. 2019, No. 32. pp. 7610 - 7618.

Bibtex

@article{5302ecf2cbc1420b9379ebfed689a2b7,
title = "Designer artificial membrane binding proteins direct stem cells to the myocardium",
abstract = "We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of achimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymersurfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approachsignificantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.",
author = "Wenjin Xiao and Green, {Tom I P} and Xiaowen Liang and {Cuahtecontzi Delint}, Rosalia and Guillaume Perry and Roberts, {Michael S} and {Le Vay}, Kris and Catherine Back and Raimondo Ascione and Haolu Wang and Paul Race and Adam Perriman",
year = "2019",
month = "7",
day = "3",
doi = "10.1039/C9SC02650A",
language = "English",
volume = "2019",
pages = "7610 -- 7618",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "32",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Designer artificial membrane binding proteins direct stem cells to the myocardium

AU - Xiao, Wenjin

AU - Green, Tom I P

AU - Liang, Xiaowen

AU - Cuahtecontzi Delint, Rosalia

AU - Perry, Guillaume

AU - Roberts, Michael S

AU - Le Vay, Kris

AU - Back, Catherine

AU - Ascione, Raimondo

AU - Wang, Haolu

AU - Race, Paul

AU - Perriman, Adam

PY - 2019/7/3

Y1 - 2019/7/3

N2 - We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of achimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymersurfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approachsignificantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

AB - We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of achimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymersurfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approachsignificantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

U2 - 10.1039/C9SC02650A

DO - 10.1039/C9SC02650A

M3 - Article

VL - 2019

SP - 7610

EP - 7618

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 32

ER -