Designing better enzymes: Insights from directed evolution

Research output: Contribution to journalArticle (Academic Journal)peer-review

33 Citations (Scopus)
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De novo enzymes can be created by computational design and directed evolution. Here, we review recent insights into the origins of catalytic power in evolved designer enzymes to pinpoint opportunities for next-generation designs: Evolution precisely organizes active sites, introduces catalytic H-bonding networks, invokes electrostatic catalysis, and creates dynamical networks embedding the active site in a reactive protein scaffold. Such insights foster our fundamental knowledge of enzyme catalysis and fuel the future design of tailor-made enzymes.
Original languageEnglish
Pages (from-to)212-218
Number of pages7
JournalCurrent Opinion in Structural Biology
Early online date29 Jan 2021
Publication statusPublished - 1 Apr 2021


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