Detection of a protein conformational equilibrium by electrospray ionisation-ion mobility-mass spectrometry

Matthew Jenner, Jacqueline Ellis, Wei Cheng Huang, Emma Lloyd Raven, Gordon C.K. Roberts, Neil J. Oldham*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

44 Citations (Scopus)

Abstract

Conforming to convention: Evidence for compact and extended forms of cytochrome P450 reductase, believed to exist in dynamic equilibrium in solution, is found in the gas phase by using ion mobility spectrometry. The relative abundance of the two conformations (see picture) can be influenced by the ionic strength of the solution from which they are electrosprayed.

Original languageEnglish
Pages (from-to)8291-8294
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number36
DOIs
Publication statusPublished - 29 Aug 2011

Keywords

  • cytochrome P450 reductase
  • gas-phase structure
  • ion mobility spectrometry
  • mass spectrometry
  • protein conformation

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