Detection of Chlamydophila felis and Feline Herpesvirus by
Multiplex Real-Time PCR Analysis

CR Helps; NA Reeves; K Egan; PE Howard; DA Harbour

doi:10.1128/JCM.41.6.2734-2736.2003

Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

CR Helps, NA Reeves, K Egan, PE Howard, DA Harbour

Research output: Contribution to journal › Article (Academic Journal) › peer-review

70 Citations (Scopus)

Abstract

Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

Translated title of the contribution	Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis
Original language	English
Pages (from-to)	2734 - 2736
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	41
Issue number	6
DOIs	https://doi.org/10.1128/JCM.41.6.2734-2736.2003
Publication status	Published - Jun 2003

Bibliographical note

Publisher: American Society for Microbiology

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Cite this

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title = "Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis",

abstract = "Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.",

author = "CR Helps and NA Reeves and K Egan and PE Howard and DA Harbour",

note = "Publisher: American Society for Microbiology",

year = "2003",

month = jun,

doi = "10.1128/JCM.41.6.2734-2736.2003",

language = "English",

volume = "41",

pages = "2734 -- 2736",

journal = "Journal of Clinical Microbiology",

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publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

AU - Helps, CR

AU - Reeves, NA

AU - Egan, K

AU - Howard, PE

AU - Harbour, DA

N1 - Publisher: American Society for Microbiology

PY - 2003/6

Y1 - 2003/6

N2 - Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

AB - Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

U2 - 10.1128/JCM.41.6.2734-2736.2003

DO - 10.1128/JCM.41.6.2734-2736.2003

M3 - Article (Academic Journal)

SN - 1098-660X

VL - 41

SP - 2734

EP - 2736

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 6