Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility

David Bitto, David J Harvey, Steinar Halldorsson, Katie J Doores, Laura K Pritchard, Juha T Huiskonen, Thomas A Bowden, Max Crispin

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Glycan analysis of virion-derived glycoproteins is challenging due to the difficulties in glycoprotein isolation and low sample abundance. Here, we describe how ion mobility mass spectrometry can be used to obtain spectra from virion samples. We also describe how negative ion fragmentation of glycans can be used to probe structural features of virion glycans.

Original languageEnglish
Pages (from-to)93-121
Number of pages29
JournalMethods in Molecular Biology
Volume1331
DOIs
Publication statusPublished - 2015

Keywords

  • Animals
  • Cell Line
  • Cricetinae
  • Glycoproteins/chemistry
  • Glycosylation
  • Ions/chemistry
  • Polysaccharides/chemistry
  • Spectrometry, Mass, Electrospray Ionization/methods
  • Viral Proteins/chemistry

Fingerprint Dive into the research topics of 'Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility'. Together they form a unique fingerprint.

Cite this