Determination of oleanolic acid in human plasma and its association with olive oil intake in healthy Spanish adults within the EPIC Spain cohort study

Genevieve Buckland*, Antoni Pastor, Leila Lujan-Barroso, Carlos Alberto Gonzalez, Noemie Travier, Pilar Amiano, José María Huerta, Antonio Agudo, Carmen Navarro, María Dolores Chirlaque, Maria-José Sánchez, Miguel Rodríguez-Barranco, Aurelio Barricarte, Eva Ardanaz, Miren Dorronsoro, Amaia Molinuevo, José Ramón Quirós, Rafael de la Torre

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

SCOPE: Oleanolic acid (OA) is an important triterpenic compound found in olive oil, however little is known about its concentrations in human plasma. We aimed to determine plasma OA levels in a healthy Spanish population and compare them with estimates of dietary olive oil intake.

METHODS AND RESULTS: The final study sample included 141 individuals randomly selected from the European Prospective Investigation into Cancer and Nutrition Spanish cohort. Dietary olive oil intake was estimated using validated dietary history questionnaires. OA concentrations were determined in plasma (from the participants' stored blood samples) using a HPLC-MS method. Correlation coefficients between OA and olive oil intake were calculated, adjusting for center; sex; age; consumption of olives, apples, grapes, and red wine; and fasting state. The mean OA concentration in olive oil nonconsumers was 0.72 ng/mL (SD 0.82), while in the high olive oil intake group it was 1.32 ng/mL (SD 1.14). The fully adjusted partial Spearman correlations coefficients reached 0.36 (p-value < 0.001) overall, varying minimally by sex and fasting state.

CONCLUSION: This is the first study providing steady-state concentrations of triterpenes in humans. The results show that there was low-to-moderate correlation between OA concentrations and olive oil intake in this population.

Original languageEnglish
Article number1600927
Number of pages9
JournalMolecular Nutrition and Food Research
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 2017

Keywords

  • Adult
  • Aged
  • Cohort Studies
  • Female
  • Humans
  • Male
  • Middle Aged
  • Nutrition Surveys
  • Oleanolic Acid/blood
  • Olive Oil/pharmacology
  • Spain

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