Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy

R J G  CARR; J G  RARITY; A G  STANSFIELD; R G W  BROWN; D J  CLARKE; T  ATKINSON

doi:10.1016/0003-2697(88)90573-8

Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy

R J G CARR, J G RARITY, A G STANSFIELD, R G W BROWN, D J CLARKE, T ATKINSON

Department of Electrical & Electronic Engineering

Research output: Contribution to journal › Article (Academic Journal) › peer-review

13 Citations (Scopus)

Abstract

The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (<5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.

Original language	English
Pages (from-to)	492-499
Number of pages	8
Journal	Analytical Biochemistry
Volume	175
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(88)90573-8
Publication status	Published - Dec 1988

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@article{edeb0d056ef545908d78496727fe559b,

title = "Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy",

abstract = "The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (<5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.",

author = "CARR, {R J G} and RARITY, {J G} and STANSFIELD, {A G} and BROWN, {R G W} and CLARKE, {D J} and T ATKINSON",

year = "1988",

month = dec,

doi = "10.1016/0003-2697(88)90573-8",

language = "English",

volume = "175",

pages = "492--499",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy

AU - CARR, R J G

AU - RARITY, J G

AU - STANSFIELD, A G

AU - BROWN, R G W

AU - CLARKE, D J

AU - ATKINSON, T

PY - 1988/12

Y1 - 1988/12

N2 - The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (<5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.

AB - The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (<5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.

U2 - 10.1016/0003-2697(88)90573-8

DO - 10.1016/0003-2697(88)90573-8

M3 - Article (Academic Journal)

VL - 175

SP - 492

EP - 499

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -

Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy

Abstract

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