Determination of protein size in chromatography column eluants by on-line photon correlation spectroscopy

R J G CARR, J G RARITY, A G STANSFIELD, R G W BROWN, D J CLARKE, T ATKINSON

Research output: Contribution to journalArticle (Academic Journal)peer-review

12 Citations (Scopus)

Abstract

The dynamic light scattering technique of photon correlation spectroscopy has been used to determine biomacromolecule hydrodynamic radius in solutions flowing at rates similar to those experienced in liquid chromatographic separation systems. Such analyses can be performed rapidly (<5 s). The potential of the technique as an on-line noninvasive monitor for liquid chromatography is discussed.
Original languageEnglish
Pages (from-to)492-499
Number of pages8
JournalAnalytical Biochemistry
Volume175
Issue number2
DOIs
Publication statusPublished - Dec 1988

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