Determination of the absolute concentrations of monoamine oxidase A and B in human tissues

A M O'Carroll, I Tobbia, K F Tipton

Research output: Contribution to journalArticle (Academic Journal)

28 Citations (Scopus)

Abstract

The concentrations of monoamine oxidase-A and -B were determined in homogenates of human cerebral cortex, caudatus and placenta and in human platelet-rich plasma and platelet membranes by determining the specific binding of tritium-labelled pargyline. The concentrations of the two enzyme forms were similar in both human brain regions examined. Determinations of the minimum quantities of clorgyline, (-)-deprenyl, J-508 or pargyline necessary to give complete inhibition of enzyme activity was found to give overestimates of the amounts of monoamine oxidase-A and -B present due to nonspecific binding of these inhibitors.

Original languageEnglish
Pages (from-to)901-5
Number of pages5
JournalBiochemical Pharmacology
Volume38
Issue number6
Publication statusPublished - 15 Mar 1989

Keywords

  • Autopsy
  • Binding Sites
  • Blood Platelets
  • Brain
  • Clorgyline
  • Humans
  • Monoamine Oxidase
  • Monoamine Oxidase Inhibitors
  • Pargyline
  • Placenta
  • Selegiline

Fingerprint Dive into the research topics of 'Determination of the absolute concentrations of monoamine oxidase A and B in human tissues'. Together they form a unique fingerprint.

Cite this