Development and application of dengue virus reverse genetic systems

Andrew D Davidson

doi:10.1007/978-1-4939-0348-1_8

Development and application of dengue virus reverse genetic systems

Andrew D Davidson

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

The development of dengue virus "reverse genetic" systems based on full-length cDNA clones corresponding to the viral RNA genome has been an important technological platform for advancing dengue virus research. Mutations can be introduced into the genome to study their effect on virus replication and pathogenesis while attenuated or chimeric viruses can be constructed that are potential vaccine candidates. The deletion of the virus structural genes has led to the production of noninfectious, but replication competent viral subgenomes (termed replicons) that have been used to study viral replication and are useful for the screening of antiviral compounds. This article describes the development of dengue virus reverse genetic systems and protocols to manipulate the viral genome, recover infectious virus, and produce replicon-containing cell lines.

Original language	English
Pages (from-to)	113-30
Number of pages	18
Journal	Methods in Molecular Biology
Volume	1138
DOIs	https://doi.org/10.1007/978-1-4939-0348-1_8
Publication status	Published - 2014

Keywords

Animals
Base Sequence
Cell Line
DNA, Complementary
Dengue Virus
Genome, Viral
Molecular Sequence Data
Plasmids
RNA, Messenger
Replicon
Reverse Genetics

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10.1007/978-1-4939-0348-1_8

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title = "Development and application of dengue virus reverse genetic systems",

abstract = "The development of dengue virus {"}reverse genetic{"} systems based on full-length cDNA clones corresponding to the viral RNA genome has been an important technological platform for advancing dengue virus research. Mutations can be introduced into the genome to study their effect on virus replication and pathogenesis while attenuated or chimeric viruses can be constructed that are potential vaccine candidates. The deletion of the virus structural genes has led to the production of noninfectious, but replication competent viral subgenomes (termed replicons) that have been used to study viral replication and are useful for the screening of antiviral compounds. This article describes the development of dengue virus reverse genetic systems and protocols to manipulate the viral genome, recover infectious virus, and produce replicon-containing cell lines.",

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PY - 2014

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N2 - The development of dengue virus "reverse genetic" systems based on full-length cDNA clones corresponding to the viral RNA genome has been an important technological platform for advancing dengue virus research. Mutations can be introduced into the genome to study their effect on virus replication and pathogenesis while attenuated or chimeric viruses can be constructed that are potential vaccine candidates. The deletion of the virus structural genes has led to the production of noninfectious, but replication competent viral subgenomes (termed replicons) that have been used to study viral replication and are useful for the screening of antiviral compounds. This article describes the development of dengue virus reverse genetic systems and protocols to manipulate the viral genome, recover infectious virus, and produce replicon-containing cell lines.

AB - The development of dengue virus "reverse genetic" systems based on full-length cDNA clones corresponding to the viral RNA genome has been an important technological platform for advancing dengue virus research. Mutations can be introduced into the genome to study their effect on virus replication and pathogenesis while attenuated or chimeric viruses can be constructed that are potential vaccine candidates. The deletion of the virus structural genes has led to the production of noninfectious, but replication competent viral subgenomes (termed replicons) that have been used to study viral replication and are useful for the screening of antiviral compounds. This article describes the development of dengue virus reverse genetic systems and protocols to manipulate the viral genome, recover infectious virus, and produce replicon-containing cell lines.

KW - Animals

KW - Base Sequence

KW - Cell Line

KW - DNA, Complementary

KW - Dengue Virus

KW - Genome, Viral

KW - Molecular Sequence Data

KW - Plasmids

KW - RNA, Messenger

KW - Replicon

KW - Reverse Genetics

U2 - 10.1007/978-1-4939-0348-1_8

DO - 10.1007/978-1-4939-0348-1_8

M3 - Article (Academic Journal)

C2 - 24696334

VL - 1138

SP - 113

EP - 130

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

Development and application of dengue virus reverse genetic systems

Abstract

Keywords

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