Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Grainne Neary*, Ashley Blom, Anna Shiel, Gabrielle Wheway, Jason Mansell

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)
213 Downloads (Pure)

Abstract

There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents to encourage superior integration of the implants into host bone. One such agent is (3S) 1-fluoro-3-hydroxy-4-(oleoyloxy) butyl- 1-phosphonate (FHBP), a phosphatase-resistant lysophosphatidic acid (LPA) analogue. We investigated the effect of an FHBP-HA coating on the maturation of human (MG63) osteoblast-like cells. Optimal coating conditions were identified and cell maturation on modified and unmodified, control HA surfaces was assessed. Stress tests were performed to evaluate coating survivorship after exposure to mechanical and thermal insults that are routinely encountered in the clinical environment. MG63 maturation was found to be 3 times greater on FHBP-modified HA compared to controls (p <0.0001). There was no significant loss of coating bioactivity after autoclaving (P= 0.9813) although functionality declined by 67% after mechanical cleaning and reuse (p<0.0001). The bioactivity of modified disks was significantly greater than that of controls following storage for up to 6 months (p<0.001). Herein we demonstrate that HA can be functionalised with FHBP in a facile, scalable manner and that this novel surface has the capacity to enhance osteoblast maturation. Improving the biological performance of HA in a bone regenerative setting could be realised through the simple conjugation of bioactive LPA species in the future.
Original languageEnglish
Article number122
Number of pages10
JournalJournal of Materials Science: Materials in Medicine
Volume29
Issue number8
Early online date21 Jul 2018
DOIs
Publication statusPublished - Aug 2018

Structured keywords

  • Centre for Surgical Research

Keywords

  • Hydroxyapatite
  • Lysophosphatidic acid
  • Biomaterial functionalisation
  • Bone graft
  • vitamin D

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