Development and evaluation of an enzyme-linked immunosorbent assay (ELISA) for the determination of the trypanocidal drug homidium in serum of treated cattle

GA Murilla*, MC Eisler, AS Peregrine, JM Ndung'u, PH Holmes

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

7 Citations (Scopus)

Abstract

Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of 0.1 ng homidium per mt serum. Homidium levels were measurable in serum of cattle for over 2 months following administration of a single intramuscular (i.m.) dose at 1 mg/kg bodyweight. The level of sensitivity afforded by these assays makes them potentially useful tools in the pharmacokinetic evaluation of homidium and for invetigating drug resistance or causes of drug failure, Assay 2 was chosen as being most suitable for further studies.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalJournal of Veterinary Pharmacology and Therapeutics
Volume22
Issue number5
Publication statusPublished - Oct 1999

Keywords

  • PHARMACOKINETICS
  • ISOMETAMIDIUM
  • BROMIDE
  • KENYA

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