Abstract
Two enzyme-linked immunosorbent assays (ELISA) for the determination of homidium in serum of treated cattle have been developed and evaluated. One is a direct competition (Assay 1) and the other an indirect competition assay (Assay 2). Both assays are highly sensitive with a limit of detection of 0.1 ng homidium per mt serum. Homidium levels were measurable in serum of cattle for over 2 months following administration of a single intramuscular (i.m.) dose at 1 mg/kg bodyweight. The level of sensitivity afforded by these assays makes them potentially useful tools in the pharmacokinetic evaluation of homidium and for invetigating drug resistance or causes of drug failure, Assay 2 was chosen as being most suitable for further studies.
| Original language | English |
|---|---|
| Pages (from-to) | 301-307 |
| Number of pages | 7 |
| Journal | Journal of Veterinary Pharmacology and Therapeutics |
| Volume | 22 |
| Issue number | 5 |
| Publication status | Published - Oct 1999 |
Keywords
- PHARMACOKINETICS
- ISOMETAMIDIUM
- BROMIDE
- KENYA