Development of a filter to prevent infections with spore-forming bacteria in injecting drug users

Nour Alhusein, Jenny Scott, Barbara Kasprzyk-Hordern, Albert Bolhuis*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

3 Citations (Scopus)

Abstract

Background: In heroin injectors, there have been a number of outbreaks caused by spore-forming bacteria, causing serious infections such as anthrax or botulism. These are, most likely, caused by injecting contaminated heroin, and our aim was to develop a filter that efficiently removes these bacteria and is also likely to be acceptable for use by people who inject drugs (i.e. quick, simple and not spoil the hit). Methods: A prototype filter was designed and different filter membranes were tested to assess the volume of liquid retained, filtration time and efficiency of the filter at removing bacterial spores. Binding of active ingredients of heroin to different types of membrane filters was determined using a highly sensitive analytical chemistry technique. Results: Heroin samples that were tested contained up to 580 bacteria per gramme, with the majority being Bacillus spp., which are spore-forming soil bacteria. To remove these bacteria, a prototype filter was designed to fit insulin-type syringes, which are commonly used by people who inject drugs (PWIDs). Efficient filtration of heroin samples was achieved by combining a prefilter to remove particles and a 0.22 μm filter to remove bacterial spores. The most suitable membrane was polyethersulfone (PES). This membrane had the shortest filtration time while efficiently removing bacterial spores. No or negligible amounts of active ingredients in heroin were retained by the PES membrane. Conclusions: This study successfully produced a prototype filter designed to filter bacterial spores from heroin samples. Scaled up production could produce an effective harm reduction tool, especially during outbreaks such as occurred in Europe in 2009/10 and 2012.

Original languageEnglish
Article number33
JournalHarm Reduction Journal
Volume13
Issue number1
DOIs
Publication statusPublished - 1 Dec 2016

Bibliographical note

Funding Information:
This study was funded by the Medical Research Council (GB) through the Confidence in Concept scheme. We thank Mr Paul Frith from the University workshop for manufacturing the prototype filter device, Dr Maria Dolores Camacho-Muñoz for helping with the chemical analysis, Ms Jolyene Alphonso for performing some of the preliminary experiments and Mr Andrew Preston and Mr Nick Wilson from Exchange Supplies Ltd for the discussions and providing syringes and Stericup packs. The work was funded by the Medical Research Council (GB). The funder had no role in the design of the study, data collection and analysis, nor in the writing of the manuscript.

Funding Information:
This study was funded by the Medical Research Council (GB) through the Confidence in Concept scheme. We thank Mr Paul Frith from the University workshop for manufacturing the prototype filter device, Dr Maria Dolores Camacho-Muñoz for helping with the chemical analysis, Ms Jolyene Alphonso for performing some of the preliminary experiments and Mr Andrew Preston and Mr Nick Wilson from Exchange Supplies Ltd for the discussions and providing syringes and Stericup packs.

Publisher Copyright:
© 2016 The Author(s).

Keywords

  • Anthrax
  • Bacterial spores
  • Filter
  • Heroin
  • PWIDs

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