Development of a minimal KASP marker panel for distinguishing genotypes in apple collections

Mark Winfield, Amanda Burridge, Matthew Ordidge, Helen Harper, Paul Wilkinson, Danny Thorogood, Liz Copas, Keith Edwards, Gary Barker

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Accurate identification of named accessions in germplasm collections is extremely important, especially for vegetatively propagated crops which are expensive to maintain. Thus, an inexpensive, reliable, and rapid genotyping method is essential because it avoids the need for laborious and time-consuming morphological comparisons. Single Nucleotide Polymorphism (SNP) marker panels containing large numbers of SNPs have been developed for many crop species, but such panels are much too large for basic cultivar identification. Here, we have identified a minimum set of SNP markers sufficient to distinguish apple cultivars held in the English and Welsh national collections providing a cheaper and automatable alternative to the markers currently used by the community. We show that SNP genotyping with a small set of well selected markers is equally efficient as microsatellites for the identification of apple cultivars and has the added advantage of automation and reduced cost when screening large numbers of samples.

Original languageEnglish
Pages (from-to)e0242940
JournalPLoS ONE
Volume15
Issue number11
DOIs
Publication statusPublished - 30 Nov 2020

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