Dexamethasone arterializes venous endothelial cells by inducing mitogen-activated protein kinase phosphatase-1: A novel antiinflammatory treatment for vein grafts?

Mustafa Zakkar, Le Anh Luong, Hera Chaudhury, Oeyvind Ruud, Prakash P. Punjabi, Jon R. Anderson, John W. Mullholand, Anne T. Clements, Rob Krams, Nicholas Foin, Thanos Athanasiou, Edward L S Leen, Justin C. Mason, Dorian O. Haskard, Paul C. Evans

Research output: Contribution to journalArticle (Academic Journal)

18 Citations (Scopus)

Abstract

Background-Vein grafting in coronary artery surgery is complicated by a high restenosis rate resulting from the development of vascular inflammation, intimal hyperplasia, and accelerated atherosclerosis. In contrast, arterial grafts are relatively resistant to these processes. Vascular inflammation is regulated by signaling intermediaries, including p38 mitogen-activated protein (MAP) kinase, that trigger endothelial cell (EC) expression of chemokines (eg, interleukin-8, monocyte chemotactic protein-1) and other proinflammatory molecules. Here, we have tested the hypothesis that p38 MAP kinase activation in response to arterial shear stress (flow) may occur more readily in venous ECs, leading to greater proinflammatory activation. Methods and Results-Comparative reverse-transcriptase polymerase chain reaction and Western blotting revealed that arterial shear stress induced p38-dependent expression of monocyte chemotactic protein-1 and interleukin-8 in porcine jugular vein ECs. In contrast, porcine aortic ECs were protected from shear stress-induced expression of p38-dependent chemokines as a result of rapid induction of MAP kinase phosphatase-1. However, we observed with both cultured porcine jugular vein ECs and perfused veins that venous ECs can be protected by brief treatment with dexamethasone, which induced MAP kinase phosphatase-1 to suppress proinflammatory activation. Conclusions-Arterial but not venous ECs are protected from proinflammatory activation in response to short-term exposure to high shear stress by the induction of MAP kinase phosphatase-1. Dexamethasone pretreatment arterializes venous ECs by inducing MAP kinase phosphatase-1 and may protect veins from inflammation.

Original languageEnglish
Pages (from-to)524-532
Number of pages9
JournalCirculation
Volume123
Issue number5
DOIs
Publication statusPublished - 8 Feb 2011

Keywords

  • endothelial shear stress
  • inflammation
  • inflammatory activation
  • MAP kinase

Fingerprint Dive into the research topics of 'Dexamethasone arterializes venous endothelial cells by inducing mitogen-activated protein kinase phosphatase-1: A novel antiinflammatory treatment for vein grafts?'. Together they form a unique fingerprint.

Cite this