Diabetes Antibody Standardization Program: evaluation of assays for autoantibodies to glutamic acid decarboxylase and islet antigen-2

C Torn, PW Mueller, M Schlosser, E Bonifacio, PJ Bingley, Laboratories Participating

Research output: Contribution to journalArticle (Academic Journal)peer-review

191 Citations (Scopus)

Abstract

Aims/hypothesis Islet autoantibodies are important in diabetes classification and risk assessment, and as endpoints in observational studies. The Diabetes Autoantibody Standardization Program (DASP) aims to improve and standardise measurement of autoantibodies associated with type 1 diabetes. We report results for glutamic acid decarboxylase autoantibodies (GADA) and islet antigen-2 autoantibodies (IA-2A) from three DASP workshops (2002–2005). Methods Up to 60 laboratories in 18 countries participated in each workshop. Participants received coded serum aliquots from 50 patients with newly diagnosed type 1 diabetes (median age 18 years, range 9–35 years) and 100 blood donor controls. Results were analysed using receiver operator characteristic (ROC) curves with sensitivity adjusted to 95% specificity in workshop controls. Results GADA assays performed well in all three workshops (median area under the ROC curve [AUC] 0.94; interquartile range 0.91–0.95) and performance was similar to DASP 2000. Performance of IA-2A assays improved over the workshop programme. Median AUC was 0.81 (interquartile range 0.79–0.83) in DASP 2002, 0.82 (interquartile range 0.78–0.84) in 2003, and 0.85 (interquartile range 0.82–0.87) in 2005 (p 
Translated title of the contributionDiabetes Antibody Standardization Program: evaluation of assays for autoantibodies to glutamic acid decarboxylase and islet antigen-2
Original languageEnglish
Pages (from-to)846 - 852
Number of pages7
JournalDiabetologia
Volume51
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint Dive into the research topics of 'Diabetes Antibody Standardization Program: evaluation of assays for autoantibodies to glutamic acid decarboxylase and islet antigen-2'. Together they form a unique fingerprint.

Cite this