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Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies

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Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies. / Schlosser, M; Mueller, PW; Törn, C; Bonifacio, E; Bingley, PJ; Participating, Laboratories.

In: Diabetologia, Vol. 53(12), 12.2010, p. 2611 - 2620.

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Schlosser, M, Mueller, PW, Törn, C, Bonifacio, E, Bingley, PJ & Participating, L 2010, 'Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies', Diabetologia, vol. 53(12), pp. 2611 - 2620. https://doi.org/10.1007/s00125-010-1915-5

APA

Schlosser, M., Mueller, PW., Törn, C., Bonifacio, E., Bingley, PJ., & Participating, L. (2010). Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies. Diabetologia, 53(12), 2611 - 2620. https://doi.org/10.1007/s00125-010-1915-5

Vancouver

Schlosser M, Mueller PW, Törn C, Bonifacio E, Bingley PJ, Participating L. Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies. Diabetologia. 2010 Dec;53(12):2611 - 2620. https://doi.org/10.1007/s00125-010-1915-5

Author

Schlosser, M ; Mueller, PW ; Törn, C ; Bonifacio, E ; Bingley, PJ ; Participating, Laboratories. / Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies. In: Diabetologia. 2010 ; Vol. 53(12). pp. 2611 - 2620.

Bibtex

@article{7a36e85f1bfd41e8a9f7768d8a46a279,
title = "Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies",
abstract = "AIMS/HYPOTHESIS: Insulin autoantibodies (IAA) are important in type 1 diabetes risk assessment. However, their determination varies more between laboratories than other diabetes autoantibodies. The Diabetes Antibody Standardization Program (DASP) aims to improve and standardise measurement of autoantibodies associated with type 1 diabetes. We report the results of measurement of IAA from DASP workshops in 2002, 2003 and 2005. METHODS: Up to 32 laboratories in 14 countries participated in each workshop. Aliquots of coded sera from 50 patients with newly diagnosed type 1 diabetes and 100 blood donor controls were circulated to participating laboratories. Reported results were analysed using receiver operator characteristic (ROC) curves. We compared concordance of antibody levels by ranking, IAA and insulin antibody (IA) indices and units derived from an IA standard curve. RESULTS: In all three workshops IAA assay performance had improved compared with DASP 2000. The median area under the ROC curve was 0.73 in DASP 2002, 0.78 in 2003 and 0.80 in 2005 (p = 0.0012), and median laboratory-assigned sensitivity was 26{\%} in 2002, 36{\%} in 2003 and 45{\%} in 2005 (p ",
author = "M Schlosser and PW Mueller and C T{\"o}rn and E Bonifacio and PJ Bingley and Laboratories Participating",
year = "2010",
month = "12",
doi = "10.1007/s00125-010-1915-5",
language = "English",
volume = "53(12)",
pages = "2611 -- 2620",
journal = "Diabetologia",
issn = "0012-186X",
publisher = "Springer Berlin Heidelberg",

}

RIS - suitable for import to EndNote

TY - JOUR

T1 - Diabetes Antibody Standardization Program: evaluation of assays for insulin autoantibodies

AU - Schlosser, M

AU - Mueller, PW

AU - Törn, C

AU - Bonifacio, E

AU - Bingley, PJ

AU - Participating, Laboratories

PY - 2010/12

Y1 - 2010/12

N2 - AIMS/HYPOTHESIS: Insulin autoantibodies (IAA) are important in type 1 diabetes risk assessment. However, their determination varies more between laboratories than other diabetes autoantibodies. The Diabetes Antibody Standardization Program (DASP) aims to improve and standardise measurement of autoantibodies associated with type 1 diabetes. We report the results of measurement of IAA from DASP workshops in 2002, 2003 and 2005. METHODS: Up to 32 laboratories in 14 countries participated in each workshop. Aliquots of coded sera from 50 patients with newly diagnosed type 1 diabetes and 100 blood donor controls were circulated to participating laboratories. Reported results were analysed using receiver operator characteristic (ROC) curves. We compared concordance of antibody levels by ranking, IAA and insulin antibody (IA) indices and units derived from an IA standard curve. RESULTS: In all three workshops IAA assay performance had improved compared with DASP 2000. The median area under the ROC curve was 0.73 in DASP 2002, 0.78 in 2003 and 0.80 in 2005 (p = 0.0012), and median laboratory-assigned sensitivity was 26% in 2002, 36% in 2003 and 45% in 2005 (p 

AB - AIMS/HYPOTHESIS: Insulin autoantibodies (IAA) are important in type 1 diabetes risk assessment. However, their determination varies more between laboratories than other diabetes autoantibodies. The Diabetes Antibody Standardization Program (DASP) aims to improve and standardise measurement of autoantibodies associated with type 1 diabetes. We report the results of measurement of IAA from DASP workshops in 2002, 2003 and 2005. METHODS: Up to 32 laboratories in 14 countries participated in each workshop. Aliquots of coded sera from 50 patients with newly diagnosed type 1 diabetes and 100 blood donor controls were circulated to participating laboratories. Reported results were analysed using receiver operator characteristic (ROC) curves. We compared concordance of antibody levels by ranking, IAA and insulin antibody (IA) indices and units derived from an IA standard curve. RESULTS: In all three workshops IAA assay performance had improved compared with DASP 2000. The median area under the ROC curve was 0.73 in DASP 2002, 0.78 in 2003 and 0.80 in 2005 (p = 0.0012), and median laboratory-assigned sensitivity was 26% in 2002, 36% in 2003 and 45% in 2005 (p 

U2 - 10.1007/s00125-010-1915-5

DO - 10.1007/s00125-010-1915-5

M3 - Article

VL - 53(12)

SP - 2611

EP - 2620

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

ER -