In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.
|Translated title of the contribution||Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction|
|Pages (from-to)||8 - 13|
|Number of pages||6|
|Journal||Journal of Feline Medicine and Surgery|
|Publication status||Published - Feb 2007|