Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

MDG Pinches, CR Helps, TJ Gruffydd-Jones, K Egan, O Jarret, S Tasker

Research output: Contribution to journalArticle (Academic Journal)peer-review

26 Citations (Scopus)

Abstract

In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.
Translated title of the contributionDiagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction
Original languageEnglish
Pages (from-to)8 - 13
Number of pages6
JournalJournal of Feline Medicine and Surgery
Volume9 (1)
DOIs
Publication statusPublished - Feb 2007

Bibliographical note

Publisher: Elsevier

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