Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

MDG Pinches; CR Helps; TJ Gruffydd-Jones; K Egan; O Jarret; S Tasker

doi:10.1016/j.jfms.2006.05.008

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

MDG Pinches, CR Helps, TJ Gruffydd-Jones, K Egan, O Jarret, S Tasker

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Abstract

In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.

Translated title of the contribution	Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction
Original language	English
Pages (from-to)	8 - 13
Number of pages	6
Journal	Journal of Feline Medicine and Surgery
Volume	9 (1)
DOIs	https://doi.org/10.1016/j.jfms.2006.05.008
Publication status	Published - Feb 2007

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Publisher: Elsevier

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title = "Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction",

abstract = "In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.",

author = "MDG Pinches and CR Helps and TJ Gruffydd-Jones and K Egan and O Jarret and S Tasker",

note = "Publisher: Elsevier",

year = "2007",

month = feb,

doi = "10.1016/j.jfms.2006.05.008",

language = "English",

volume = "9 (1)",

pages = "8 -- 13",

journal = "Journal of Feline Medicine and Surgery",

issn = "1532-2750",

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TY - JOUR

T1 - Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

AU - Pinches, MDG

AU - Helps, CR

AU - Gruffydd-Jones, TJ

AU - Egan, K

AU - Jarret, O

AU - Tasker, S

N1 - Publisher: Elsevier

PY - 2007/2

Y1 - 2007/2

N2 - In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.

AB - In this paper the design and use of a semi-quantitative real-time polymerase chain reaction assay (RT-PCR) for feline leukaemia virus (FeLV) provirus is described. Its performance is evaluated against established methods of FeLV diagnosis, including virus isolation and enzyme-linked immunoassay (ELISA) in a population of naturally infected cats. The RT-PCR assay is found to have both a high sensitivity (0.92) and specificity (0.99) when examined by expectation maximisation methods and is also able to detect a large number of cats with low FeLV proviral loads that were negative by other conventional test methods.

U2 - 10.1016/j.jfms.2006.05.008

DO - 10.1016/j.jfms.2006.05.008

M3 - Article (Academic Journal)

C2 - 16861024

SN - 1532-2750

VL - 9 (1)

SP - 8

EP - 13

JO - Journal of Feline Medicine and Surgery

JF - Journal of Feline Medicine and Surgery

ER -

Diagnosis of feline leukaemia virus infection by semi-quantitative real-time polymerase chain reaction

Abstract

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