Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Tarcisio Brignoli; Mario Recker; Winnie W.Y. Lee; Tim Dong; Ranjeet Bhamber; Mahableshwar Albur; Philip Williams; Andrew W. Dowsey; Ruth C. Massey

doi:10.1099/mic.0.001223

Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Tarcisio Brignoli, Mario Recker, Winnie W.Y. Lee, Tim Dong, Ranjeet Bhamber, Mahableshwar Albur, Philip Williams, Andrew W. Dowsey, Ruth C. Massey^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.

Original language	English
Article number	001223
Number of pages	6
Journal	Microbiology
Volume	168
Issue number	8
DOIs	https://doi.org/10.1099/mic.0.001223
Publication status	Published - 23 Aug 2022

Bibliographical note

Funding Information:
This work was funded by a Wellcome Trust funded Investigator awarded to RCM (Grant reference number: 212258/Z/18/Z), The Showering Fund award (SF124) awarded to MA, an MRC grant (MR/T005408/1) awarded to PW, and the Health Data Research UK South-West Better Care Partnership AMR Site Project, lead AWD. Health Data Research UK is an initiative funded by UK Research and Innovation, Department of Health and Social Care (England) and the devolved administrations, and leading medical research charities.

Publisher Copyright:
© 2022, Microbiology Society. All rights reserved.

Keywords

bacteraemia
Agr
MALDI-TOF MS diagnosis
Staphylococcus aureus
toxicity

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title = "Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome",

abstract = "Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.",

keywords = "bacteraemia, Agr, MALDI-TOF MS diagnosis, Staphylococcus aureus, toxicity",

author = "Tarcisio Brignoli and Mario Recker and Lee, {Winnie W.Y.} and Tim Dong and Ranjeet Bhamber and Mahableshwar Albur and Philip Williams and Dowsey, {Andrew W.} and Massey, {Ruth C.}",

note = "Funding Information: This work was funded by a Wellcome Trust funded Investigator awarded to RCM (Grant reference number: 212258/Z/18/Z), The Showering Fund award (SF124) awarded to MA, an MRC grant (MR/T005408/1) awarded to PW, and the Health Data Research UK South-West Better Care Partnership AMR Site Project, lead AWD. Health Data Research UK is an initiative funded by UK Research and Innovation, Department of Health and Social Care (England) and the devolved administrations, and leading medical research charities. Publisher Copyright: {\textcopyright} 2022, Microbiology Society. All rights reserved.",

year = "2022",

month = aug,

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language = "English",

volume = "168",

journal = "Microbiology",

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AU - Brignoli, Tarcisio

AU - Recker, Mario

AU - Lee, Winnie W.Y.

AU - Dong, Tim

AU - Bhamber, Ranjeet

AU - Albur, Mahableshwar

AU - Williams, Philip

AU - Dowsey, Andrew W.

AU - Massey, Ruth C.

N1 - Funding Information: This work was funded by a Wellcome Trust funded Investigator awarded to RCM (Grant reference number: 212258/Z/18/Z), The Showering Fund award (SF124) awarded to MA, an MRC grant (MR/T005408/1) awarded to PW, and the Health Data Research UK South-West Better Care Partnership AMR Site Project, lead AWD. Health Data Research UK is an initiative funded by UK Research and Innovation, Department of Health and Social Care (England) and the devolved administrations, and leading medical research charities. Publisher Copyright: © 2022, Microbiology Society. All rights reserved.

PY - 2022/8/23

Y1 - 2022/8/23

N2 - Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.

AB - Staphylococcus aureus bacteraemia (SAB) is a major cause of blood-stream infection (BSI) in both healthcare and community settings. While the underlying comorbidities of a patient significantly contributes to their susceptibility to and outcome following SAB, recent studies show the importance of the level of cytolytic toxin production by the infecting bacterium. In this study we demonstrate that this cytotoxicity can be determined directly from the diagnostic MALDI-TOF mass spectrum generated in a routine diagnostic laboratory. With further development this information could be used to guide the management and improve the outcomes for SAB patients.

KW - bacteraemia

KW - Agr

KW - MALDI-TOF MS diagnosis

KW - Staphylococcus aureus

KW - toxicity

U2 - 10.1099/mic.0.001223

DO - 10.1099/mic.0.001223

M3 - Article (Academic Journal)

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AN - SCOPUS:85137124120

SN - 1350-0872

VL - 168

JO - Microbiology

JF - Microbiology

IS - 8

M1 - 001223

ER -

Diagnostic MALDI-TOF MS can differentiate between high and low toxic Staphylococcus aureus bacteraemia isolates as a predictor of patient outcome

Abstract

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Keywords

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