Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices

DA Holman; M Feligioni; JM Henley

doi:10.1016/j.neuropharm.2006.05.022

Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices

DA Holman, M Feligioni, JM Henley

Research output: Contribution to journal › Article (Academic Journal) › peer-review

22 Citations (Scopus)

Abstract

AMPAR trafficking is crucial for the expression of certain forms of synaptic plasticity. Here, using surface biotinylation of hippocampal slices and subsequent synaptosome isolation we assessed AMPAR surface expression in synaptosomes following NMDA-evoked long-term depression (NMDA-LTD). Surface levels of GluR1, GluR2 and GluR3 in synaptosomes were markedly reduced 90 min after NMDA-LTD induction. Consistent with endocytosis and degradation, whole-cell surface and total expression levels of GluR2 and GluR3 were also reduced. In contrast, whole-cell surface levels of GluR1 were unaltered at 90 min suggesting that AMPARs with different subunit composition are redistributed to different non-synaptic compartments following LTD induction in acute hippocampal slices.

Translated title of the contribution	Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices
Original language	English
Pages (from-to)	92 - 99
Number of pages	8
Journal	Neuropharmacology
Volume	52 (1)
DOIs	https://doi.org/10.1016/j.neuropharm.2006.05.022
Publication status	Published - Jan 2007

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Publisher: Elsevier

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10.1016/j.neuropharm.2006.05.022

http://dx.doi.org/10.1016/j.neuropharm.2006.05.022

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MECHANISMS CONTROLLING THE NUMBER AND LOCATION OF SYNAPTIC AMPR'S
Henley, J. M. (Principal Investigator)
1/01/08 → 1/01/13
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title = "Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices",

abstract = "AMPAR trafficking is crucial for the expression of certain forms of synaptic plasticity. Here, using surface biotinylation of hippocampal slices and subsequent synaptosome isolation we assessed AMPAR surface expression in synaptosomes following NMDA-evoked long-term depression (NMDA-LTD). Surface levels of GluR1, GluR2 and GluR3 in synaptosomes were markedly reduced 90 min after NMDA-LTD induction. Consistent with endocytosis and degradation, whole-cell surface and total expression levels of GluR2 and GluR3 were also reduced. In contrast, whole-cell surface levels of GluR1 were unaltered at 90 min suggesting that AMPARs with different subunit composition are redistributed to different non-synaptic compartments following LTD induction in acute hippocampal slices.",

author = "DA Holman and M Feligioni and JM Henley",

note = "Publisher: Elsevier",

year = "2007",

month = jan,

doi = "10.1016/j.neuropharm.2006.05.022",

language = "English",

volume = "52 (1)",

pages = "92 -- 99",

journal = "Neuropharmacology",

issn = "1873-7064",

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TY - JOUR

T1 - Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices

AU - Holman, DA

AU - Feligioni, M

AU - Henley, JM

N1 - Publisher: Elsevier

PY - 2007/1

Y1 - 2007/1

N2 - AMPAR trafficking is crucial for the expression of certain forms of synaptic plasticity. Here, using surface biotinylation of hippocampal slices and subsequent synaptosome isolation we assessed AMPAR surface expression in synaptosomes following NMDA-evoked long-term depression (NMDA-LTD). Surface levels of GluR1, GluR2 and GluR3 in synaptosomes were markedly reduced 90 min after NMDA-LTD induction. Consistent with endocytosis and degradation, whole-cell surface and total expression levels of GluR2 and GluR3 were also reduced. In contrast, whole-cell surface levels of GluR1 were unaltered at 90 min suggesting that AMPARs with different subunit composition are redistributed to different non-synaptic compartments following LTD induction in acute hippocampal slices.

AB - AMPAR trafficking is crucial for the expression of certain forms of synaptic plasticity. Here, using surface biotinylation of hippocampal slices and subsequent synaptosome isolation we assessed AMPAR surface expression in synaptosomes following NMDA-evoked long-term depression (NMDA-LTD). Surface levels of GluR1, GluR2 and GluR3 in synaptosomes were markedly reduced 90 min after NMDA-LTD induction. Consistent with endocytosis and degradation, whole-cell surface and total expression levels of GluR2 and GluR3 were also reduced. In contrast, whole-cell surface levels of GluR1 were unaltered at 90 min suggesting that AMPARs with different subunit composition are redistributed to different non-synaptic compartments following LTD induction in acute hippocampal slices.

U2 - 10.1016/j.neuropharm.2006.05.022

DO - 10.1016/j.neuropharm.2006.05.022

M3 - Article (Academic Journal)

C2 - 16815481

SN - 1873-7064

VL - 52 (1)

SP - 92

EP - 99

JO - Neuropharmacology

JF - Neuropharmacology

ER -

Differential redistribution of native AMPA receptor complexes following LTD induction in acute hippocampal slices

Abstract

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MECHANISMS CONTROLLING THE NUMBER AND LOCATION OF SYNAPTIC AMPR'S

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