It has been reported that Stenotrophomonas maltophilia L1 and L2 beta-lactamase expression is coordinated. We have isolated S. maltophilia mutants where (i) L1 is constitutively hyper-expressed and L2 is inducible; (ii) L2 is hyper-expressed and L1 is inducible; and (iii) L1 and L2 are constitutively hyper-expressed. The frequency of isolating type 1 and 2 mutants is c. 10(-7), indicating that promoter mutations are probably not involved and providing strong evidence that L1 and L2 expression is not, after all, coordinated. The frequency of isolating type 3 mutants is c. 10(-9), however, implying that there is a significant overlap between the regulatory mechanisms.
|Translated title of the contribution||Differential regulation of L1 and L2 β-lactamase expression in Stenotrophomonas maltophilia|
|Pages (from-to)||387 - 389|
|Number of pages||3|
|Journal||Journal of Antimicrobial Chemotherapy|
|Publication status||Published - Feb 2002|
Bibliographical notePublisher: Oxford University Press
Other: Authors Ford & von Heldreich were UG students