TY - CHAP
T1 - Differential Scanning Calorimetry to Quantify Heat-Induced Aggregation in Concentrated Protein Solutions
AU - Jacobs, Matthew R.
AU - Grace, Mark
AU - Blumlein, Alice
AU - McManus, Jennifer J.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Differential scanning calorimetry (DSC) is an important technique to measure the thermodynamics of protein unfolding (or folding). Information including the temperature for the onset of unfolding, the melt transition temperature (Tm), enthalpy of unfolding (ΔH), and refolding index (RI) are useful for evaluating the heat stability of proteins for a range of biochemical, structural biology, industrial, and pharmaceutical applications. We describe a procedure for careful sample preparation of proteins for DSC measurements and data analysis to determine a range of thermodynamic parameters. In particular, we highlight a measure of protein refolding following complete thermal denaturation (RI), which quantifies the proportion of protein lost to irreversible aggregation after thermal denaturation.
AB - Differential scanning calorimetry (DSC) is an important technique to measure the thermodynamics of protein unfolding (or folding). Information including the temperature for the onset of unfolding, the melt transition temperature (Tm), enthalpy of unfolding (ΔH), and refolding index (RI) are useful for evaluating the heat stability of proteins for a range of biochemical, structural biology, industrial, and pharmaceutical applications. We describe a procedure for careful sample preparation of proteins for DSC measurements and data analysis to determine a range of thermodynamic parameters. In particular, we highlight a measure of protein refolding following complete thermal denaturation (RI), which quantifies the proportion of protein lost to irreversible aggregation after thermal denaturation.
KW - Differential scanning calorimetry
KW - Enthalpy
KW - Melt transition temperature
KW - Protein aggregation
KW - Refolding index
KW - Thermal denaturation
UR - http://www.scopus.com/inward/record.url?scp=85070214168&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-9678-0_9
DO - 10.1007/978-1-4939-9678-0_9
M3 - Chapter in a book
C2 - 31342423
AN - SCOPUS:85070214168
T3 - Methods in Molecular Biology
SP - 117
EP - 129
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -