Directly from the source: endogenous preparations of molecular machines

Pablo Mesa, Aurélien Deniaud, Guillermo Montoya, Christiane Schaffitzel

Research output: Contribution to journalArticle (Academic Journal)

9 Citations (Scopus)

Abstract

Purification from a source enriched in large macromolecular machines with basic cellular function is still the method of choice in many cases. Such complexes occur in sufficiently high copy numbers in the cell and can be isolated using classical protein purification protocols. Although advanced DNA recombinant technologies and sophisticated overexpression strategies are available, many complexes like the ribosome, RNA polymerase II and membrane protein complexes involved in photosynthesis or in oxidative phosphorylation can only be purified from a rich source. Here, we review recent accomplishments and limitations in applying this strategy.

Original languageEnglish
Pages (from-to)319-25
Number of pages7
JournalCurrent Opinion in Structural Biology
Volume23
Issue number3
DOIs
Publication statusPublished - Jun 2013

Bibliographical note

Copyright © 2013. Published by Elsevier Ltd.

Keywords

  • Analytic Sample Preparation Methods
  • Animals
  • Bacteria
  • Crystallization
  • DNA, Recombinant
  • Databases, Protein
  • Humans
  • Multiprotein Complexes
  • Protein Conformation
  • Recombinant Proteins
  • Yeasts

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