Dissection of the ozone-induced calcium signature

H Clayton, M R Knight, H Knight, M R McAinsh, A M Hetherington

Research output: Contribution to journalArticle (Academic Journal)

93 Citations (Scopus)

Abstract

The cellular responses of plants to numerous extracellular stimuli are mediated by transient elevations in the concentration of cytosolic free calcium ([Ca2+](cyt)). We have addressed the question of how cells can use this apparently ubiquitous system to initiate so many specific and appropriate end responses. We show that the pollutant gas ozone elicits a biphasic Ca2+ response in intact Arabidopsis plants and a subsequent increase in expression of the gene encoding the antioxidant defence enzyme glutathione-S-transferase (GST). The second of the two [Ca2+](cyt) peaks, but not the first, could be eliminated either by pre-treatment of plants with lanthanum chloride, or by reducing the duration of ozone fumigation. Under these conditions, ozone-induced GST expression was abolished. These data provide a functional dissection of the ozone Ca2+ signalling pathway and indicate that the second ozone-induced [Ca2+](cyt) peak provides the necessary information to direct expression of GST.

Original languageEnglish
Pages (from-to)575-579
Number of pages5
JournalPlant Journal
Volume17
Issue number5
Publication statusPublished - Mar 1999

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