Dissemination of genetically diverse NDM-1, -5, -7 producing-Gram-negative pathogens isolated from pediatric patients in Pakistan

Muhammad Usman Qamar, Timothy R Walsh, Mark A Toleman, Jonathan M Tyrrell, Sidrah Saleem, Ali Aboklaish, Shah Jahan

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Aim: To determine the prevalence of New Delhi metallo-β-lactamase (NDM)-producing Gram-negative pathogens isolated from children's samples. Materials & methods: Carbapenem-resistant clinical isolates (n = 117) were confirmed by VITEK® 2 compact system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing. MIC (μg/ml) of various antibiotics was determined by VITEK 2 compact system. Molecular characterization of the isolates was performed by PCR, DNA sequencing, PFGE and DNA hybridization. Results: Out of 117 carbapenemase producers, 37 (31.6%) and 29 (24.7%) were Klebsiella pneumoniae and Acinetobacter baumannii, respectively. 72 (61.5%) isolates were NDM positive and among these 60, 9 and 3 were NDM-1, -5 and -7, respectively. Majority of the NDM-producing K. pneumoniae belonged to ST11 and ST273 while most of the Escherichia coli belonged to ST405 and ST101. blaNDM were mainly located on 150kb plasmids. MIC displayed high resistance against β-lactams drugs including carbapenems, and the most sensitive drugs were tigecycline and colistin. Conclusion: Dissemination of blaNDM-producing pathogens, particularly in children clinical settings, is a matter of great public health concern.

Original languageEnglish
Pages (from-to)691-704
Number of pages14
JournalFuture Microbiology
Volume14
DOIs
Publication statusPublished - May 2019

Keywords

  • Acinetobacter baumannii/drug effects
  • Anti-Bacterial Agents/pharmacology
  • Bacterial Proteins/biosynthesis
  • Child
  • DNA, Bacterial/analysis
  • Drug Resistance, Multiple, Bacterial/genetics
  • Escherichia coli/drug effects
  • Female
  • Gene Expression Profiling
  • Gram-Negative Bacteria/drug effects
  • Gram-Negative Bacterial Infections/epidemiology
  • Humans
  • Klebsiella pneumoniae/drug effects
  • Male
  • Microbial Sensitivity Tests
  • Molecular Epidemiology
  • Multilocus Sequence Typing
  • Pakistan/epidemiology
  • Plasmids
  • Sequence Analysis, DNA
  • beta-Lactamases/biosynthesis

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