DNA methylation and transcription onset in the brain

Renaud Massart; Matthew Suderman; Valerie Mongrain; Moshe Szyf

doi:10.2217/epi-2016-0184

DNA methylation and transcription onset in the brain

Renaud Massart, Matthew Suderman, Valerie Mongrain, Moshe Szyf

Research output: Contribution to journal › Article (Academic Journal) › peer-review

5 Citations (Scopus)

Abstract

The goal of this study was to test the state of methylation of transcription start positions in DNA that are actively involved in transcription. Materials & methods: We used sequential ChIP-bisulfite-sequencing with an antibody to RNpolII-PS5 to map the state of methylation of actively transcribing transcription start sites (TSS). Results: TSS that RNApolII-PS5 physically bind to, are ubiquitously unmethylated. TSS that appear to be both heavily methylated and transcriptionally active are truly a mixture of unmethylated TSS with bound RNApolII-PS5 in some nuclei and unbound methylated TSS in other nuclei. Conclusion: TSS DNA methylation is universally inconsistent with transcription onset and could therefore serve as a digital count of the fraction of nuclei with methylation-silenced TSS.

Original language	English
Pages (from-to)	797-809
Number of pages	13
Journal	Epigenomics
Volume	9
Issue number	6
DOIs	https://doi.org/10.2217/epi-2016-0184
Publication status	Published - 18 May 2017

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Dr Matthew J Suderman
- matthew.suderman@bristol.ac.uk
- Bristol Medical School (PHS) - Lecturer in Epigenetic Epidemiology
- Bristol Population Health Science Institute
- MRC Integrative Epidemiology Unit
Person: Academic , Member

Cite this

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title = "DNA methylation and transcription onset in the brain",

abstract = "The goal of this study was to test the state of methylation of transcription start positions in DNA that are actively involved in transcription. Materials & methods: We used sequential ChIP-bisulfite-sequencing with an antibody to RNpolII-PS5 to map the state of methylation of actively transcribing transcription start sites (TSS). Results: TSS that RNApolII-PS5 physically bind to, are ubiquitously unmethylated. TSS that appear to be both heavily methylated and transcriptionally active are truly a mixture of unmethylated TSS with bound RNApolII-PS5 in some nuclei and unbound methylated TSS in other nuclei. Conclusion: TSS DNA methylation is universally inconsistent with transcription onset and could therefore serve as a digital count of the fraction of nuclei with methylation-silenced TSS.",

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AU - Suderman, Matthew

AU - Mongrain, Valerie

AU - Szyf, Moshe

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AB - The goal of this study was to test the state of methylation of transcription start positions in DNA that are actively involved in transcription. Materials & methods: We used sequential ChIP-bisulfite-sequencing with an antibody to RNpolII-PS5 to map the state of methylation of actively transcribing transcription start sites (TSS). Results: TSS that RNApolII-PS5 physically bind to, are ubiquitously unmethylated. TSS that appear to be both heavily methylated and transcriptionally active are truly a mixture of unmethylated TSS with bound RNApolII-PS5 in some nuclei and unbound methylated TSS in other nuclei. Conclusion: TSS DNA methylation is universally inconsistent with transcription onset and could therefore serve as a digital count of the fraction of nuclei with methylation-silenced TSS.

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DO - 10.2217/epi-2016-0184

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DNA methylation and transcription onset in the brain

Abstract

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Dr Matthew J Suderman

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