Abstract
The goal of this study was to test the state of methylation of transcription start positions in DNA that are actively involved in transcription. Materials & methods: We used sequential ChIP-bisulfite-sequencing with an antibody to RNpolII-PS5 to map the state of methylation of actively transcribing transcription start sites (TSS). Results: TSS that RNApolII-PS5 physically bind to, are ubiquitously unmethylated. TSS that appear to be both heavily methylated and transcriptionally active are truly a mixture of unmethylated TSS with bound RNApolII-PS5 in some nuclei and unbound methylated TSS in other nuclei. Conclusion: TSS DNA methylation is universally inconsistent with transcription onset and could therefore serve as a digital count of the fraction of nuclei with methylation-silenced TSS.
Original language | English |
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Pages (from-to) | 797-809 |
Number of pages | 13 |
Journal | Epigenomics |
Volume | 9 |
Issue number | 6 |
DOIs | |
Publication status | Published - 18 May 2017 |
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Dr Matthew J Suderman
- Bristol Medical School (PHS) - Associate Professor in Molecular Epidemiology
- Bristol Population Health Science Institute
- MRC Integrative Epidemiology Unit
Person: Academic , Member