TY - JOUR
T1 - DNA Methylome Marks of Exposure to Particulate Matter at Three Time Points in Early Life
AU - Plusquin, Michelle
AU - Chadeau-Hyam, Marc
AU - Ghantous, Akram
AU - Alfano, Rossella
AU - Bustamante, Mariona
AU - Chatzi, Leda
AU - Cuenin, Cyrille
AU - Gulliver, John
AU - Herceg, Zdenko
AU - Kogevinas, Manolis
AU - Nawrot, Tim S.
AU - Pizzi, Costanza
AU - Porta, Daniela
AU - Relton, Caroline L.
AU - Richiardi, Lorenzo
AU - Robinson, Oliver
AU - Sunyer, Jordi
AU - Vermeulen, Roel
AU - Vriens, Annette
AU - Vrijheid, Martine
AU - Henderson, John
AU - Vineis, Paolo
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Maternal exposure to airborne particulate matter (PM) has been associated with restricted fetal growth and reduced birthweight. Here, we performed methylome-wide analyses of cord and children's blood DNA in relation to residential exposure to PM smaller than 10 μm (PM10). This study included participants of the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC, cord blood, n = 780; blood at age 7, n = 757 and age 15-17, n = 850) and the EXPOsOMICS birth cohort consortium including cord blood from ENVIRONAGE (n = 197), INMA (n = 84), Piccolipiù (n = 99) and Rhea (n = 75). We could not identify significant CpG sites, by meta-analyzing associations between maternal PM10 exposure during pregnancy and DNA methylation in cord blood, nor by studying DNA methylation and concordant annual exposure at 7 and 15-17 years. The CpG cg21785536 was inversely associated with PM10 exposure using a longitudinal model integrating the three studied age groups (-1.2% per 10 μg/m3; raw p-value = 3.82 × 10-8). Pathway analyses on the corresponding genes of the 100 strongest associated CpG sites of the longitudinal model revealed enriched pathways relating to the GABAergic synapse, p53 signaling and NOTCH1. We provided evidence that residential PM10 exposure in early life affects methylation of the CpG cg21785536 located on the EGF Domain Specific O-Linked N-Acetylglucosamine Transferase gene.
AB - Maternal exposure to airborne particulate matter (PM) has been associated with restricted fetal growth and reduced birthweight. Here, we performed methylome-wide analyses of cord and children's blood DNA in relation to residential exposure to PM smaller than 10 μm (PM10). This study included participants of the Avon Longitudinal Study of Pregnancy and Childhood (ALSPAC, cord blood, n = 780; blood at age 7, n = 757 and age 15-17, n = 850) and the EXPOsOMICS birth cohort consortium including cord blood from ENVIRONAGE (n = 197), INMA (n = 84), Piccolipiù (n = 99) and Rhea (n = 75). We could not identify significant CpG sites, by meta-analyzing associations between maternal PM10 exposure during pregnancy and DNA methylation in cord blood, nor by studying DNA methylation and concordant annual exposure at 7 and 15-17 years. The CpG cg21785536 was inversely associated with PM10 exposure using a longitudinal model integrating the three studied age groups (-1.2% per 10 μg/m3; raw p-value = 3.82 × 10-8). Pathway analyses on the corresponding genes of the 100 strongest associated CpG sites of the longitudinal model revealed enriched pathways relating to the GABAergic synapse, p53 signaling and NOTCH1. We provided evidence that residential PM10 exposure in early life affects methylation of the CpG cg21785536 located on the EGF Domain Specific O-Linked N-Acetylglucosamine Transferase gene.
UR - http://www.scopus.com/inward/record.url?scp=85046395494&partnerID=8YFLogxK
U2 - 10.1021/acs.est.7b06447
DO - 10.1021/acs.est.7b06447
M3 - Article (Academic Journal)
C2 - 29597345
AN - SCOPUS:85046395494
VL - 52
SP - 5427
EP - 5437
JO - Environmental Science and Technology
JF - Environmental Science and Technology
SN - 0013-936X
IS - 9
ER -