Abstract
Progress in whole-genome sequencing using short-read (e.g., <150 bp), next-generation sequencing technologies has reinvigorated interest in high-resolution physical mapping to fill technical gaps that are not well addressed by sequencing. Here, we report two technical advances in DNA nanotechnology and single-molecule genomics: (1) we describe a labeling technique (CRISPR-Cas9 nanoparticles) for high-speed AFM-based physical mapping of DNA and (2) the first successful demonstration of using DVD optics to image DNA molecules with high-speed AFM. As a proof of principle, we used this new “nanomapping” method to detect and map precisely BCL2–IGH translocations present in lymph node biopsies of follicular lymphoma patents. This HS-AFM “nanomapping” technique can be complementary to both sequencing and other physical mapping approaches.
Original language | English |
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Pages (from-to) | 1665-1674 |
Number of pages | 9 |
Journal | Nature Communications |
Volume | 8 |
DOIs | |
Publication status | Published - 21 Nov 2017 |
Keywords
- DNA
- CRISPR–Cas
- CRISPR
- AFM
- high-speed AFM
- High-speed atomic force microscopy
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Dr Oliver D Payton
- Interface Analysis Centre - Senior Research Associate
- The Bristol Centre for Nanoscience and Quantum Information
- Applied Nonlinear Mathematics
Person: Academic , Member