Does inflammation induced by ultraviolet B and heat rekindling alter pain-related behaviour in rats?

Melissa I Smith, Collette Taylor, Nirosha S Weerasinghe, Stella Koutsikou, Bridget M Lumb, Jo C Murrell

Research output: Contribution to journalArticle (Academic Journal)peer-review

2 Citations (Scopus)
253 Downloads (Pure)

Abstract

Objective

To investigate whether induction of the ultraviolet B and heat rekindling (UVB/HR) model alters burrowing behaviour in rats.

Study design

Randomized, blinded, prospective experimental study.

Animals

Sixteen adult male Wistar rats weighing 250–300 g.

Methods

In the UVB/HR group (n = 8), UV irradiation was delivered to the heel area of the right plantar pelvic limb paw at a dose of 1000 mJ cm−2, using a narrow-band UVB light source. Twenty-four hours later, heat rekindling was performed by placement of a feedback-controlled thermode set at a constant temperature of 45 °C over the area of UVB irradiation for 5 minutes. Both interventions were carried out under pentobarbital anaesthesia. The ‘sham’ group (n = 8) was anaesthetized only. In the burrowing test, rats were housed singly for 2 hours in cages furnished with a burrow filled with sand. The amount of sand remaining in the burrow after 2 hours was weighed and the amount displaced from the burrow calculated. The burrowing test was carried out for two consecutive days prior to UVB irradiation (day 0), on day 1 prior to HR, on days 2 and 3 after UVB exposure and at equivalent time points in the sham group.

Results

Rats in the sham group burrowed means (SD) of 2429 (73) g and 2358 (124) g of sand on days −2 and 3, respectively, while those in the UVB/HR group burrowed 2460 (26) and 2419 (58) g on days −2 and 3, respectively. There was no significant effect of treatment on the amount of sand burrowed at any time point.

Conclusions and clinical relevance

Pain associated with UVB/HR model induction is below the threshold required to affect rat burrowing behaviour and therefore questions the face validity of UVB/HR as a translational model of inflammatory pain.

Original languageEnglish
Pages (from-to)579–585
Number of pages7
JournalVeterinary Anaesthesia and Analgesia
Volume43
Issue number5
Early online date12 Feb 2016
DOIs
Publication statusPublished - Sep 2016

Keywords

  • burrowing
  • heat rekindling
  • pain
  • rat
  • ultraviolet B

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