Skip to content

Domestication of campylobacter jejuni NCTC 11168

Research output: Contribution to journalArticle

  • Ben Pascoe
  • Lisa K Williams
  • Jessica K Calland
  • Guillaume Meric
  • Matthew D Hitchings
  • Myles Dyer
  • Tristan A Cogan
  • et al.
Original languageEnglish
Number of pages12
JournalMicrobial Genomics
Issue number7
Early online date1 Jul 2019
DateAccepted/In press - 3 Jun 2019
DateE-pub ahead of print - 1 Jul 2019
DatePublished (current) - 16 Jul 2019


Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the
reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C. jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

    Research areas

  • Campylobacter, genomics, microbial evolution, culture collections

Download statistics

No data available



  • Full-text PDF (final published version)

    Rights statement: This is the final published version of the article (version of record). It first appeared online via Microbiology Society at . Please refer to any applicable terms of use of the publisher.

    Final published version, 1.03 MB, PDF document

    Licence: CC BY-NC


View research connections

Related faculties, schools or groups