Dual Action of Lysophosphatidate-Functionalised Titanium: Interactions with Human (MG63) Osteoblasts and Methicillin Resistant Staphylococcus aureus.

Mett Skindersoe, Karen Krogfelt, Ashley Blom, J Zhang, G Jiang, Glenn Prestwich, Jason Mansell

Research output: Contribution to journalArticle (Academic Journal)

6 Citations (Scopus)

Abstract

Abstract
Titanium (Ti) is a widely used material for surgical implants; total joint replacements (TJRs), screws and plates for fixing bones and dental implants are forged from Ti. Whilst Ti integrates well into host tissue approximately 10% of TJRs will fail in the lifetime of the patient through a process known as aseptic loosening. These failures necessitate revision arthroplasties which are more complicated and costly than the initial procedure. Finding ways of enhancing early (osseo)integration of TJRs is therefore highly desirable and continues to represent a research priority in current biomaterial design. One way of realising improvements in implant quality is to coat the Ti surface with small biological agents known to support human osteoblast formation and maturation at Ti surfaces. Lysophosphatidic acid (LPA) and certain LPA analogues offer potential solutions as Ti coatings in reducing aseptic loosening. Herein we present evidence for the successful bio-functionalisation of Ti using LPA. This modified Ti surface heightened the maturation of human osteoblasts, as supported by increased expression of alkaline phosphatase. These functionalised surfaces also deterred the attachment and growth of Staphylococcus aureus, a bacterium often associated with implant failures through sepsis. Collectively we provide evidence for the fabrication of a dual-action Ti surface finish, a highly desirable feature towards the development of next-generation implantable devices.
Original languageEnglish
Article number26605796
Pages (from-to)e0143509
JournalPLoS ONE
Volume10
Issue number11
Publication statusPublished - 25 Nov 2015

Structured keywords

  • Centre for Surgical Research

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    Skindersoe, M., Krogfelt, K., Blom, A., Zhang, J., Jiang, G., Prestwich, G., & Mansell, J. (2015). Dual Action of Lysophosphatidate-Functionalised Titanium: Interactions with Human (MG63) Osteoblasts and Methicillin Resistant Staphylococcus aureus. PLoS ONE, 10(11), e0143509. [26605796].