Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis

Gioacchino Luca Losacco; Raffeal Bennett; Imad A. Haidar Ahmad; Rodell C. Barrientos; Jimmy O. Dasilva; Yuyang Dong; Alexander W. Schuppe; Zeshu Wang; Sheenagh Aiken; Ian Mangion; Varinder K. Aggarwal; Erik L. Regalado

doi:10.1002/anie.202208854

Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis

Gioacchino Luca Losacco, Raffeal Bennett, Imad A. Haidar Ahmad, Rodell C. Barrientos, Jimmy O. Dasilva, Yuyang Dong, Alexander W. Schuppe, Zeshu Wang, Sheenagh Aiken, Ian Mangion, Varinder K. Aggarwal, Erik L. Regalado

School of Chemistry

Research output: Contribution to journal › Article (Academic Journal) › peer-review

13 Citations (Scopus)

Abstract

Generality in analytical chemistry can be manifested in impactful platforms that can streamline modern organic synthesis and biopharmaceutical processes. We herein introduce a hybrid separation technique named Dual-Gradient Unified Chromatography (DGUC), which is built upon an automated dynamic modulation of CO2, organic modifier, and water blends. This concept enables simultaneous multicomponent analysis of both small and large molecules across a wide polarity range in single experimental runs. After a careful investigation of its fundamental aspects, a DGUC-DAD-MS screening workflow that combines multiple orthogonal column and mobile phase choices across a far-reaching universal elution profile is also reported. The power of this framework is demonstrated with new analytical applications guiding academic and industrial laboratories in the development of new (bio)pharmaceutical targets (e.g. synthetic intermediates, nucleosides, cyclic and linear peptides, proteins, antibody drug conjugates).

Original language	English
Article number	e202208854
Journal	Angewandte Chemie - International Edition
Volume	61
Issue number	45
Early online date	16 Sept 2022
DOIs	https://doi.org/10.1002/anie.202208854
Publication status	E-pub ahead of print - 16 Sept 2022

Bibliographical note

Funding Information:
The authors would like to thank Prof. Stephen L. Buchwald for insightful discussion and asymmetric catalysis support. We also thank Dr. Peter Ratsep for his input on the SFC system. The MRL Postdoctoral Research Fellow Program is acknowledged for financial support provided by a fellowship (G. L. L.). Research reported in this publication was also supported by the Arnold and Mabel Beckman Foundation for a postdoctoral fellowship (A. W. S.) and the National Institutes of Health (R35‐GM122483).

Publisher Copyright:
© 2022 Wiley-VCH GmbH.

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Losacco, G. L., Bennett, R., Ahmad, I. A. H., Barrientos, R. C., Dasilva, J. O., Dong, Y., Schuppe, A. W., Wang, Z., Aiken, S., Mangion, I., Aggarwal, V. K., & Regalado, E. L. (2022). Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis. Angewandte Chemie - International Edition, 61(45), Article e202208854. Advance online publication. https://doi.org/10.1002/anie.202208854

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title = "Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis",

abstract = "Generality in analytical chemistry can be manifested in impactful platforms that can streamline modern organic synthesis and biopharmaceutical processes. We herein introduce a hybrid separation technique named Dual-Gradient Unified Chromatography (DGUC), which is built upon an automated dynamic modulation of CO2, organic modifier, and water blends. This concept enables simultaneous multicomponent analysis of both small and large molecules across a wide polarity range in single experimental runs. After a careful investigation of its fundamental aspects, a DGUC-DAD-MS screening workflow that combines multiple orthogonal column and mobile phase choices across a far-reaching universal elution profile is also reported. The power of this framework is demonstrated with new analytical applications guiding academic and industrial laboratories in the development of new (bio)pharmaceutical targets (e.g. synthetic intermediates, nucleosides, cyclic and linear peptides, proteins, antibody drug conjugates).",

author = "Losacco, {Gioacchino Luca} and Raffeal Bennett and Ahmad, {Imad A. Haidar} and Barrientos, {Rodell C.} and Dasilva, {Jimmy O.} and Yuyang Dong and Schuppe, {Alexander W.} and Zeshu Wang and Sheenagh Aiken and Ian Mangion and Aggarwal, {Varinder K.} and Regalado, {Erik L.}",

note = "Funding Information: The authors would like to thank Prof. Stephen L. Buchwald for insightful discussion and asymmetric catalysis support. We also thank Dr. Peter Ratsep for his input on the SFC system. The MRL Postdoctoral Research Fellow Program is acknowledged for financial support provided by a fellowship (G. L. L.). Research reported in this publication was also supported by the Arnold and Mabel Beckman Foundation for a postdoctoral fellowship (A. W. S.) and the National Institutes of Health (R35‐GM122483). Publisher Copyright: {\textcopyright} 2022 Wiley-VCH GmbH.",

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Losacco, GL, Bennett, R, Ahmad, IAH, Barrientos, RC, Dasilva, JO, Dong, Y, Schuppe, AW, Wang, Z, Aiken, S, Mangion, I, Aggarwal, VK & Regalado, EL 2022, 'Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis', Angewandte Chemie - International Edition, vol. 61, no. 45, e202208854. https://doi.org/10.1002/anie.202208854

TY - JOUR

T1 - Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis

AU - Losacco, Gioacchino Luca

AU - Bennett, Raffeal

AU - Ahmad, Imad A. Haidar

AU - Barrientos, Rodell C.

AU - Dasilva, Jimmy O.

AU - Dong, Yuyang

AU - Schuppe, Alexander W.

AU - Wang, Zeshu

AU - Aiken, Sheenagh

AU - Mangion, Ian

AU - Aggarwal, Varinder K.

AU - Regalado, Erik L.

N1 - Funding Information: The authors would like to thank Prof. Stephen L. Buchwald for insightful discussion and asymmetric catalysis support. We also thank Dr. Peter Ratsep for his input on the SFC system. The MRL Postdoctoral Research Fellow Program is acknowledged for financial support provided by a fellowship (G. L. L.). Research reported in this publication was also supported by the Arnold and Mabel Beckman Foundation for a postdoctoral fellowship (A. W. S.) and the National Institutes of Health (R35‐GM122483). Publisher Copyright: © 2022 Wiley-VCH GmbH.

PY - 2022/9/16

Y1 - 2022/9/16

N2 - Generality in analytical chemistry can be manifested in impactful platforms that can streamline modern organic synthesis and biopharmaceutical processes. We herein introduce a hybrid separation technique named Dual-Gradient Unified Chromatography (DGUC), which is built upon an automated dynamic modulation of CO2, organic modifier, and water blends. This concept enables simultaneous multicomponent analysis of both small and large molecules across a wide polarity range in single experimental runs. After a careful investigation of its fundamental aspects, a DGUC-DAD-MS screening workflow that combines multiple orthogonal column and mobile phase choices across a far-reaching universal elution profile is also reported. The power of this framework is demonstrated with new analytical applications guiding academic and industrial laboratories in the development of new (bio)pharmaceutical targets (e.g. synthetic intermediates, nucleosides, cyclic and linear peptides, proteins, antibody drug conjugates).

AB - Generality in analytical chemistry can be manifested in impactful platforms that can streamline modern organic synthesis and biopharmaceutical processes. We herein introduce a hybrid separation technique named Dual-Gradient Unified Chromatography (DGUC), which is built upon an automated dynamic modulation of CO2, organic modifier, and water blends. This concept enables simultaneous multicomponent analysis of both small and large molecules across a wide polarity range in single experimental runs. After a careful investigation of its fundamental aspects, a DGUC-DAD-MS screening workflow that combines multiple orthogonal column and mobile phase choices across a far-reaching universal elution profile is also reported. The power of this framework is demonstrated with new analytical applications guiding academic and industrial laboratories in the development of new (bio)pharmaceutical targets (e.g. synthetic intermediates, nucleosides, cyclic and linear peptides, proteins, antibody drug conjugates).

U2 - 10.1002/anie.202208854

DO - 10.1002/anie.202208854

M3 - Article (Academic Journal)

C2 - 36111975

SN - 1433-7851

VL - 61

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 45

M1 - e202208854

ER -

Dual‐Gradient Unified Chromatography: A New Paradigm for Versatility in Simultaneous Multicomponent Analysis

Abstract

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