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Dynamic compound-specific stable isotope probing of the soil microbial biomass

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Original languageEnglish
Title of host publicationMicrobial biomass
Subtitle of host publicationa paradigm shift in terrestrial biogeochemistry
EditorsKevin R Tate
Publisher or commissioning bodyWorld Scientific Publishing Co.
Number of pages34
ISBN (Print)9781786341303
DateIn preparation - 2017
DatePublished (current) - 8 Apr 2017


The concept of following the assimilation of stable, isotopically labelled substrates
into newly synthesised soil microbial biomass biochemicals (= biomarkers) was
first demonstrated nearly 20 years ago in studies of unculturable soil methanotrophs (Radajewski et al., 2000; Bull et al., 2000). These landmark papers laid the foundations for an approach that has come to be generally known as stable isotope probing (SIP). The major advantage of SIP in studying the soil microbial
biomass lies in its effectiveness for investigating unculturable soil microorganisms,
that is, the vast majority of the soil bacteria, actinomycetes, fungi, algae and

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