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Ecological and geographical speciation in Lucilia bufonivora: The evolution of amphibian obligate parasitism

Research output: Contribution to journalArticle

  • Gerardo Arias Robledo
  • Richard L Wall
  • Krzysztof Szpila
  • Danny Shpeley
  • Terry Whitworth
  • Tariq Stark
  • Andrew King
  • Jamie Stevens
Original languageEnglish
Pages (from-to)218-230
Number of pages13
JournalInternational Journal for Parasitology: Parasites and Wildlife
Volume10
Early online date22 Sep 2019
DOIs
DateAccepted/In press - 20 Sep 2019
DateE-pub ahead of print - 22 Sep 2019
DatePublished (current) - 1 Dec 2019

Abstract

Lucilia (Diptera: Calliphoridae) is a genus of blowflies comprised largely of saprophagous and facultative parasites of livestock. Lucilia bufonivora, however, exhibits a unique form of obligate parasitism of amphibians, typically affecting wild hosts. The evolutionary route by which amphibian myiasis arose, however, is not well understood due to the low phylogenetic resolution in existing nuclear DNA phylogenies. Furthermore, the timing of when specificity for amphibian hosts arose in L. bufonivora is also unknown. In addition, this species was recently reported for the first time in North America (Canada) and, to date, no molecular studies have analysed the evolutionary relationships between individuals from Eastern and Western hemispheres. To provide broader insights into the evolution of the amphibian parasitic life history trait and to estimate when the trait first arose, a time-scaled phylogeny was inferred from a concatenated data set comprising mtDNA, nDNA and non-coding rDNA (COX1, per and ITS2 respectively). Specimens from Canada, the UK, Poland, Switzerland, the Netherlands and Germany were analysed, as well as individuals from its sister taxa, the saprophage Lucilia silvarum and a Nearctic species also implicated in amphibian myiasis, Lucilia elongata. Obligate amphibian parasitism appears to have arisen ~4 mya, likely as a result of niche displacement of a saprophagous/facultative parasite ancestor. Consistent paraphyly of L. bufonivora with respect to L. elongata across single-gene phylogenies and high mtDNA genetic distances between Nearctic and Palearctic individuals suggest on-going cryptic speciation facilitated by geographical isolation. These findings suggest that recent reports of L. bufonivora in the Nearctic do not constitute a recent introduction, but instead suggest that it remained unrecorded due to taxonomic confusion and low abundance. This is the first study to confirm the involvement of L. bufonivora in amphibian myiasis in Canada using DNA-based identification methods.

    Research areas

  • Amphibian parasitism, Blowfly, Host specialisation, Lucilia, Myiasis, Obligate parasitism

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  • Full-text PDF (final published version)

    Rights statement: This is the final published version of the article (version of record). It first appeared online via Elsevier at https://www.sciencedirect.com/science/article/pii/S2213224419301907 . Please refer to any applicable terms of use of the publisher.

    Final published version, 1 MB, PDF document

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  • Full-text PDF (final published version)

    Rights statement: This is the final published version of the article (version of record). It first appeared online via Elsevier at https://www.sciencedirect.com/science/article/pii/S2213224419301907?via%3Dihub#!. Please refer to any applicable terms of use of the publisher.

    Final published version, 4 MB, PDF document

    Licence: CC BY

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