Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis

K. J. Allen; K. V. Tennant; S. H. Franklin

doi:10.1016/j.tvjl.2019.105405

Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis

K. J. Allen^*, K. V. Tennant, S. H. Franklin

^*Corresponding author for this work

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Abstract

Published studies vary as to whether epithelial cells are included in differential counts for tracheal wash (TW) and bronchoalveolar lavage (BAL) cytology in horses. The aim of this study was to determine whether inclusion or exclusion of epithelial cells affects interpretation of airway cytology. Using criteria of >20% TW neutrophils, >10% BAL neutrophils and/or >5% BAL mast cells to indicate airway inflammation, there was a change in categorisation from ‘normal’ to ‘abnormal’ in 21%, 4% and 8% horses, respectively, when epithelial cells were excluded from differential counts. It is recommended that future equine respiratory research studies explicitly state whether epithelial cells are included or excluded in differential counts. A consensus on epithelial cell inclusion during cytology reporting is required.

Original language	English
Article number	105405
Number of pages	2
Journal	Veterinary Journal
Volume	254
Early online date	10 Nov 2019
DOIs	https://doi.org/10.1016/j.tvjl.2019.105405
Publication status	Published - 1 Dec 2019

Keywords

Bronchoalveolar lavage
Cytology
Epithelial cell
Horse
Tracheal wash

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This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Elsevier at https://www.sciencedirect.com/science/article/pii/S1090023319301388#! . Please refer to any applicable terms of use of the publisher.
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title = "Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis",

abstract = "Published studies vary as to whether epithelial cells are included in differential counts for tracheal wash (TW) and bronchoalveolar lavage (BAL) cytology in horses. The aim of this study was to determine whether inclusion or exclusion of epithelial cells affects interpretation of airway cytology. Using criteria of >20% TW neutrophils, >10% BAL neutrophils and/or >5% BAL mast cells to indicate airway inflammation, there was a change in categorisation from {\textquoteleft}normal{\textquoteright} to {\textquoteleft}abnormal{\textquoteright} in 21%, 4% and 8% horses, respectively, when epithelial cells were excluded from differential counts. It is recommended that future equine respiratory research studies explicitly state whether epithelial cells are included or excluded in differential counts. A consensus on epithelial cell inclusion during cytology reporting is required.",

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author = "Allen, {K. J.} and Tennant, {K. V.} and Franklin, {S. H.}",

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journal = "Veterinary Journal",

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T1 - Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis

AU - Allen, K. J.

AU - Tennant, K. V.

AU - Franklin, S. H.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Published studies vary as to whether epithelial cells are included in differential counts for tracheal wash (TW) and bronchoalveolar lavage (BAL) cytology in horses. The aim of this study was to determine whether inclusion or exclusion of epithelial cells affects interpretation of airway cytology. Using criteria of >20% TW neutrophils, >10% BAL neutrophils and/or >5% BAL mast cells to indicate airway inflammation, there was a change in categorisation from ‘normal’ to ‘abnormal’ in 21%, 4% and 8% horses, respectively, when epithelial cells were excluded from differential counts. It is recommended that future equine respiratory research studies explicitly state whether epithelial cells are included or excluded in differential counts. A consensus on epithelial cell inclusion during cytology reporting is required.

AB - Published studies vary as to whether epithelial cells are included in differential counts for tracheal wash (TW) and bronchoalveolar lavage (BAL) cytology in horses. The aim of this study was to determine whether inclusion or exclusion of epithelial cells affects interpretation of airway cytology. Using criteria of >20% TW neutrophils, >10% BAL neutrophils and/or >5% BAL mast cells to indicate airway inflammation, there was a change in categorisation from ‘normal’ to ‘abnormal’ in 21%, 4% and 8% horses, respectively, when epithelial cells were excluded from differential counts. It is recommended that future equine respiratory research studies explicitly state whether epithelial cells are included or excluded in differential counts. A consensus on epithelial cell inclusion during cytology reporting is required.

KW - Bronchoalveolar lavage

KW - Cytology

KW - Epithelial cell

KW - Horse

KW - Tracheal wash

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DO - 10.1016/j.tvjl.2019.105405

M3 - Article (Academic Journal)

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AN - SCOPUS:85075010259

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VL - 254

JO - Veterinary Journal

JF - Veterinary Journal

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Effect of inclusion or exclusion of epithelial cells in equine respiratory cytology analysis

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