TY - JOUR
T1 - Effect of temperature and fixation on the optical properties of atherosclerotic tissue
T2 - a validation study of an ex-vivo whole heart cadaveric model
AU - Gnanadesigan, Muthukaruppan
AU - van Soest, Gijs
AU - White, Stephen
AU - Scoltock, Simon
AU - Ughi, Giovanni J
AU - Baumbach, Andreas
AU - van der Steen, Antonius Fw
AU - Regar, Evelyn
AU - Johnson, Thomas W
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Atherosclerotic plaque composition can be imaged using the optical attenuation coefficient derived from intravascular optical coherence tomography (OCT) data. The relation between optical properties and tissue type has been established on autopsy tissues. In this study, we validate an ex-vivo model for the effect of temperature and tissue fixation on optical parameters. We studied the optical attenuation of human coronary arteries at three temperatures, before and after formalin fixation. We developed an en-face longitudinal display of attenuation data of the OCT pullbacks. Using the unfixed, body-temperature condition image as a standard, and after extensive registration with other condition images, we quantify the differences in optical attenuation and the backscattered intensity. The results suggest that tissue fixation and temperature do not introduce systematic errors in studies of arterial optical properties.
AB - Atherosclerotic plaque composition can be imaged using the optical attenuation coefficient derived from intravascular optical coherence tomography (OCT) data. The relation between optical properties and tissue type has been established on autopsy tissues. In this study, we validate an ex-vivo model for the effect of temperature and tissue fixation on optical parameters. We studied the optical attenuation of human coronary arteries at three temperatures, before and after formalin fixation. We developed an en-face longitudinal display of attenuation data of the OCT pullbacks. Using the unfixed, body-temperature condition image as a standard, and after extensive registration with other condition images, we quantify the differences in optical attenuation and the backscattered intensity. The results suggest that tissue fixation and temperature do not introduce systematic errors in studies of arterial optical properties.
U2 - 10.1364/BOE.5.001038
DO - 10.1364/BOE.5.001038
M3 - Article (Academic Journal)
C2 - 24761287
VL - 5
SP - 1038
EP - 1049
JO - Biomedical Optical Express
JF - Biomedical Optical Express
SN - 2156-7085
IS - 4
ER -