Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens

Nitish Boodhoo; Bahram Shojadoost; Mohammadali Alizadeh; Jake Astill; Shahriar Behboudi; Shayan Sharif

doi:10.3389/fmicb.2023.1257819

Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens

Nitish Boodhoo, Bahram Shojadoost, Mohammadali Alizadeh, Jake Astill, Shahriar Behboudi, Shayan Sharif

Bristol Veterinary School

Research output: Contribution to journal › Article (Academic Journal) › peer-review

6 Citations (Scopus)

Abstract

Alterations in intestinal microbiota can modulate the developing avian intestinal immune system and, subsequently, may impact on resistance to enteric pathogens. The aim was to demonstrate that early life exposure to Lactococcus lactis, could affect either susceptibility or resistance of broilers to necrotic enteritis (NE). L. lactis NZ9000 (rL. lactis) pre-treatment at 1, 7, 14 and 21 days of age (DOA) led to a significant decrease in NE lesion scores in Clostridium perfringens infected chickens. C. perfringens Infection was associated with spatial and temporal decreases in mononuclear phagocytes and CD4+ αβ T cells. However, rL. Lactis pre-treatment and subsequent C. perfringens infection led to a significant increase in mononuclear phagocytes, CD8α + γδ T, αβ T cells (CD4+ and CD8α+) and B cells (IgM+, IgA+ and IgY+), as well as IL-12p40, IFN-γ and CD40. Differential expression of interleukin (IL)-6, IL-8, IL-10, IL-13, IL-18, IL-22, and transforming growth factor (TGF)-β were observed in L. lactis treated chickens when compared to C. perfringens infected chickens. Microbiota analysis in C. perfringens infected chickens demonstrated an increase in abundance of Bacillota, Bacteroidota, Pseudomonadota and Actinomycetota. These findings suggests that modulation of the chicken intestinal immune system by L. lactis confers partial protection 30 against NE.

Original language	English
Article number	1257819
Journal	Frontiers in Microbiology
Volume	14
DOIs	https://doi.org/10.3389/fmicb.2023.1257819
Publication status	Published - 18 Dec 2023

Bibliographical note

Funding Information:
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported with funds from the Canadian Poultry Research Council, Natural Sciences and Engineering Research Council, Ontario Research Fund-Research Excellence, the Ontario Ministry of Agriculture, Food and Rural Affairs, University of Guelph’s Food from Thought initiative, thanks to funding from the Canada First Research Excellence Fund. SB was supported by funding from the Biotechnology and Biological Sciences Research Council (award no. BBS/E/I/00001825).

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Copyright © 2023 Boodhoo, Shojadoost, Alizadeh, Astill, Behboudi and Sharif.

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@article{9f039967c9f34ac0ad28ae48844afaed,

title = "Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens",

abstract = "Alterations in intestinal microbiota can modulate the developing avian intestinal immune system and, subsequently, may impact on resistance to enteric pathogens. The aim was to demonstrate that early life exposure to Lactococcus lactis, could affect either susceptibility or resistance of broilers to necrotic enteritis (NE). L. lactis NZ9000 (rL. lactis) pre-treatment at 1, 7, 14 and 21 days of age (DOA) led to a significant decrease in NE lesion scores in Clostridium perfringens infected chickens. C. perfringens Infection was associated with spatial and temporal decreases in mononuclear phagocytes and CD4+ αβ T cells. However, rL. Lactis pre-treatment and subsequent C. perfringens infection led to a significant increase in mononuclear phagocytes, CD8α + γδ T, αβ T cells (CD4+ and CD8α+) and B cells (IgM+, IgA+ and IgY+), as well as IL-12p40, IFN-γ and CD40. Differential expression of interleukin (IL)-6, IL-8, IL-10, IL-13, IL-18, IL-22, and transforming growth factor (TGF)-β were observed in L. lactis treated chickens when compared to C. perfringens infected chickens. Microbiota analysis in C. perfringens infected chickens demonstrated an increase in abundance of Bacillota, Bacteroidota, Pseudomonadota and Actinomycetota. These findings suggests that modulation of the chicken intestinal immune system by L. lactis confers partial protection 30 against NE.",

author = "Nitish Boodhoo and Bahram Shojadoost and Mohammadali Alizadeh and Jake Astill and Shahriar Behboudi and Shayan Sharif",

note = "Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported with funds from the Canadian Poultry Research Council, Natural Sciences and Engineering Research Council, Ontario Research Fund-Research Excellence, the Ontario Ministry of Agriculture, Food and Rural Affairs, University of Guelph{\textquoteright}s Food from Thought initiative, thanks to funding from the Canada First Research Excellence Fund. SB was supported by funding from the Biotechnology and Biological Sciences Research Council (award no. BBS/E/I/00001825). Publisher Copyright: Copyright {\textcopyright} 2023 Boodhoo, Shojadoost, Alizadeh, Astill, Behboudi and Sharif.",

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doi = "10.3389/fmicb.2023.1257819",

language = "English",

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Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens. / Boodhoo, Nitish; Shojadoost, Bahram; Alizadeh, Mohammadali et al.
In: Frontiers in Microbiology, Vol. 14, 1257819, 18.12.2023.

Research output: Contribution to journal › Article (Academic Journal) › peer-review

TY - JOUR

T1 - Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens

AU - Boodhoo, Nitish

AU - Shojadoost, Bahram

AU - Alizadeh, Mohammadali

AU - Astill, Jake

AU - Behboudi, Shahriar

AU - Sharif, Shayan

N1 - Funding Information: The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported with funds from the Canadian Poultry Research Council, Natural Sciences and Engineering Research Council, Ontario Research Fund-Research Excellence, the Ontario Ministry of Agriculture, Food and Rural Affairs, University of Guelph’s Food from Thought initiative, thanks to funding from the Canada First Research Excellence Fund. SB was supported by funding from the Biotechnology and Biological Sciences Research Council (award no. BBS/E/I/00001825). Publisher Copyright: Copyright © 2023 Boodhoo, Shojadoost, Alizadeh, Astill, Behboudi and Sharif.

PY - 2023/12/18

Y1 - 2023/12/18

N2 - Alterations in intestinal microbiota can modulate the developing avian intestinal immune system and, subsequently, may impact on resistance to enteric pathogens. The aim was to demonstrate that early life exposure to Lactococcus lactis, could affect either susceptibility or resistance of broilers to necrotic enteritis (NE). L. lactis NZ9000 (rL. lactis) pre-treatment at 1, 7, 14 and 21 days of age (DOA) led to a significant decrease in NE lesion scores in Clostridium perfringens infected chickens. C. perfringens Infection was associated with spatial and temporal decreases in mononuclear phagocytes and CD4+ αβ T cells. However, rL. Lactis pre-treatment and subsequent C. perfringens infection led to a significant increase in mononuclear phagocytes, CD8α + γδ T, αβ T cells (CD4+ and CD8α+) and B cells (IgM+, IgA+ and IgY+), as well as IL-12p40, IFN-γ and CD40. Differential expression of interleukin (IL)-6, IL-8, IL-10, IL-13, IL-18, IL-22, and transforming growth factor (TGF)-β were observed in L. lactis treated chickens when compared to C. perfringens infected chickens. Microbiota analysis in C. perfringens infected chickens demonstrated an increase in abundance of Bacillota, Bacteroidota, Pseudomonadota and Actinomycetota. These findings suggests that modulation of the chicken intestinal immune system by L. lactis confers partial protection 30 against NE.

AB - Alterations in intestinal microbiota can modulate the developing avian intestinal immune system and, subsequently, may impact on resistance to enteric pathogens. The aim was to demonstrate that early life exposure to Lactococcus lactis, could affect either susceptibility or resistance of broilers to necrotic enteritis (NE). L. lactis NZ9000 (rL. lactis) pre-treatment at 1, 7, 14 and 21 days of age (DOA) led to a significant decrease in NE lesion scores in Clostridium perfringens infected chickens. C. perfringens Infection was associated with spatial and temporal decreases in mononuclear phagocytes and CD4+ αβ T cells. However, rL. Lactis pre-treatment and subsequent C. perfringens infection led to a significant increase in mononuclear phagocytes, CD8α + γδ T, αβ T cells (CD4+ and CD8α+) and B cells (IgM+, IgA+ and IgY+), as well as IL-12p40, IFN-γ and CD40. Differential expression of interleukin (IL)-6, IL-8, IL-10, IL-13, IL-18, IL-22, and transforming growth factor (TGF)-β were observed in L. lactis treated chickens when compared to C. perfringens infected chickens. Microbiota analysis in C. perfringens infected chickens demonstrated an increase in abundance of Bacillota, Bacteroidota, Pseudomonadota and Actinomycetota. These findings suggests that modulation of the chicken intestinal immune system by L. lactis confers partial protection 30 against NE.

U2 - 10.3389/fmicb.2023.1257819

DO - 10.3389/fmicb.2023.1257819

M3 - Article (Academic Journal)

C2 - 38164397

SN - 1664-302X

VL - 14

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 1257819

ER -

Effect of treatment with Lactococcus lactis NZ9000 on intestinal microbiota and mucosal immune responses against Clostridium perfringens in broiler chickens

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