Effects of heparin and derivatives on podocytes: An in vitro functional and morphological evaluation.

Olivier Delézay*, Zhiguo Hé, Odile Sabido, Sophie Hodin, Valérie Bin, Moin Saleem, Patrick Mismetti, Xavier Delavenne

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

1 Citation (Scopus)
252 Downloads (Pure)

Abstract

Unfractionated heparin (UFH) and low molecular heparin derivatives (LMWH) display numerous biological properties in addition to their anticoagulant effects. However, due to the physicochemical heterogeneity of these drugs, a better understanding concerning their effects on human cells is clearly needed. Considering that heparins are mainly excreted by the kidney, we focused our attention on the effect of UFH and LMWH on human podocytes by functional and morphological/phenotypic in vitro analyses. We demonstrated that these products differentially modulate the permeability of podocyte monolayer to albumin. The functional perturbations observed were correlated to significant cellular morphological and cytoskeletal changes, as well as a decrease in the expression of proteins involved in podocyte adherence to the extracellular matrix or intercellular interactions. This point confirms that UFH and the different LMWHs exert specific effects on podocyte permeability and underlines the need of in vitro tests to evaluate new biological nonanticoagulant properties of LMWH.
Original languageEnglish
Pages (from-to)15438-15449
Number of pages12
JournalJournal of Cellular Physiology
Volume234
Issue number9
Early online date26 Jan 2019
DOIs
Publication statusPublished - 1 Sep 2019

Keywords

  • heparin
  • low molecular heparin derivatives
  • podocyte
  • podocyte permeability

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