Effects of hypophysectomy and growth hormone administration on the mRNA levels of collagen I, III and insulin-like growth factor-I in rat skeletal muscle

V J Wilson, M Rattray, C R Thomas, B H Moreland, D Schulster

Research output: Contribution to journalArticle (Academic Journal)peer-review

8 Citations (Scopus)

Abstract

The effect of short-term treatment of normal or hypophysectomized rats with biosynthetic growth hormone (GH) was studied in extensor digitorum longus and soleus muscles. In situ hybridization revealed that in normal rats, mRNA for collagen I, collagen III and insulin-like growth factor-I (IGF-I) are expressed by fibroblasts between the muscle fibre areas and that the specificity of this location was not altered by GH administration. Hypophysectomy appeared to cause a decrease in IGF-I and decreased collagen I and III gene expression (P < 0.001, P < 0.001, respectively). GH administration seemed to increase IGF-I mRNA levels in all the animals studied. Quantitative image analysis that GH administration to hypophysectomized rats caused an increase in collagen I gene expression after 2 days (P < 0.05) and an increase in collagen III gene expression after 4 days (P < 0.05). The results indicate that the fibroblast cells are an important target for the action of GH on skeletal muscle and that the fibroblasts respond to GH by increases in the expression of mRNA for collagen I and collagen III.

Original languageEnglish
Pages (from-to)431-8
Number of pages8
JournalGrowth Hormone and IGF Research
Volume8
Issue number6
Publication statusPublished - Dec 1998

Keywords

  • Animals
  • Collagen
  • Fibroblasts
  • Gene Expression Regulation
  • Human Growth Hormone
  • Hypophysectomy
  • In Situ Hybridization
  • Insulin-Like Growth Factor I
  • Male
  • Muscle, Skeletal
  • RNA, Messenger
  • Rats
  • Rats, Sprague-Dawley
  • Reference Values
  • Transcription, Genetic
  • Journal Article
  • Research Support, Non-U.S. Gov't

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