Effects of RhoA and RhoC upon the sensitivity of prostate cancer cells to glutamine deprivation

Luciana Bueno De Paiva, Vanessa Aline Bernusso, João Agostinho Machado-Neto, Fabiola Traina, Anne Ridley, Sara Teresinha Olalla-Saad, Mariana Lazarini

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Abstract

RhoA and RhoC contribute to the regulation of glutamine metabolism, which is a crucial determinant of cell growth in some types of cancer. Here we investigated the participation of RhoA and RhoC in the response of prostate cancer cells to glutamine deprivation. We found that RhoA and RhoC activities were up- or downregulated by glutamine reduction in PC3 and LNCaP cells, which was concomitant to a reduction in cell number and proliferation. Stable overexpression of wild type RhoA or RhoC did not alter the sensitivity to glutamine deprivation. However, PC3 cells expressing dominant negative RhoAN19 or RhoCN19 mutants were more resistant to glutamine deprivation. Our results indicate that RhoA and RhoC activities could affect cancer treatments targeting the glutamine pathway.
Original languageEnglish
Number of pages8
JournalSmall GTPases
Early online date18 Nov 2018
DOIs
Publication statusE-pub ahead of print - 18 Nov 2018

Keywords

  • prostate cancer
  • Rho GTPases
  • RhoC
  • RhoA
  • LNCaP cells
  • PC3 cells
  • glutamine

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