Projects per year
Abstract
Transmembrane anion transporters (anionophores) have potential for new modes of biological activity, including therapeutic applications. In particular they might replace the activity of defective anion channels in conditions such as cystic fibrosis. However, data on the biological effects of anionophores are scarce, and it remains uncertain whether such molecules are fundamentally toxic. Here, we report a biological study of an extensive series of powerful anion carriers. Fifteen anionophores were assayed in single cells by monitoring anion transport in real time through fluorescence emission from halide-sensitive yellow fluorescent protein. A bis-(p-nitrophenyl)ureidodecalin shows especially promising activity, including deliverability, potency and persistence. Electrophysiological tests show strong effects in epithelia, close to those of natural anion channels. Toxicity assays yield negative results in three cell lines, suggesting that promotion of anion transport may not be deleterious to cells. We therefore conclude that synthetic anion carriers are realistic candidates for further investigation as treatments for cystic fibrosis.
Original language | English |
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Pages (from-to) | 24-32 |
Number of pages | 9 |
Journal | Nature Chemistry |
Volume | 8 |
Issue number | 1 |
Early online date | 2 Nov 2015 |
DOIs | |
Publication status | Published - Jan 2016 |
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Dive into the research topics of 'Efficient, non‐toxic anion transport by synthetic carriers in cells and epithelia'. Together they form a unique fingerprint.Projects
- 3 Finished
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3-month Core Capability for Chemistry Research
Crosby, J. (Principal Investigator)
1/01/13 → 1/04/13
Project: Research
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Synthetic Anionophores with Therapeutic Potential - a Coordinated Two-Centre Approach
Davis, A. P. (Principal Investigator)
10/09/12 → 9/03/16
Project: Research
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SYNTHETIC ANION CARRIERS FOR BIOMEDICAL APPLICATIONS
Davis, A. P. (Principal Investigator)
1/11/08 → 1/03/12
Project: Research
Equipment
Profiles
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Professor Anthony P Davis
- School of Chemistry - Professor of Supramolecular Chemistry
Person: Academic
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Professor David N Sheppard
- School of Physiology, Pharmacology & Neuroscience - Professor of Physiology
- Dynamic Cell Biology
Person: Academic , Member