Electrostatically gated membrane permeability in inorganic protocells

Mei Li, Rachel Harbron, Jonathan Weaver, Bernard Binks, Stephen Mann

Research output: Contribution to journalArticle (Academic Journal)

234 Citations (Scopus)
386 Downloads (Pure)

Abstract

Although several strategies are now available to produce functional microcompartments analogous to primitive cell-like structures, little progress has been made in generating protocell constructs with self-controlled membrane permeability. Here we describe the preparation of water-dispersible colloidosomes based on silica nanoparticles and delineated by a continuous semipermeable inorganic membrane capable of self-activated, electrostatically gated permeability. We use crosslinking and covalent grafting of a pH-responsive copolymer to generate an ultrathin elastic membrane that exhibits selective release and uptake of small molecules. This behaviour, which depends on the charge of the copolymer coronal layer, serves to trigger enzymatic dephosphorylation reactions specifically within the protocell aqueous interior. This system represents a step towards the design and construction of alternative types of artificial chemical cells and protocell models based on spontaneous processes of inorganic self-organization.
Original languageEnglish
Pages (from-to)529-536
Number of pages8
JournalNature Chemistry
Volume5
Issue number6
Early online date5 May 2013
DOIs
Publication statusPublished - Jun 2013

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