Enantioselective reduction of β-keto acids with engineered streptomyces coelicolor

Kevin I. Booker-Milburn*, Rebecca Gillan, Meriel Kimberley, Takaaki Taguchi, Koji Ichinose, G. Richard Stephenson, Yutaka Ebizuka, David A. Hopwood

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

8 Citations (Scopus)

Abstract

Compelling evidence for the intermediacy of the free β-keto acid 1, rather than the corresponding enzyme-bound thiolate as previously proposed, in the biosynthesis of the antibiotic actinorhodin (2) was obtained from studies of the enantioselective reduction of a range of β-keto acids by the engineered strain of S. coelicolor CH999/pIJ5675. This excellent whole-cell biotransformation system gave the desired S β-hydroxy acids with >95% ee.

Original languageEnglish
Pages (from-to)1121-1125
Number of pages5
JournalAngewandte Chemie - International Edition
Volume44
Issue number7
DOIs
Publication statusPublished - 4 Feb 2005

Keywords

  • Bioorganic chemistry
  • Biosynthesis
  • Enantioselectivity
  • Enzymes
  • Reduction

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