Endogeonous calcium buffers regulate fast exocytosis in the synaptic terminal of retinal biploar cells

J Burrone, G Neves, A Gomis, AC Cooke, L Lagnado

Research output: Contribution to journalArticle (Academic Journal)peer-review

72 Citations (Scopus)

Abstract

Calcium-triggered exocytosis at the synapse is suppressed by addition of calcium chelators, but the effects of endogenous Ca(2+) buffers have not been tested. We find that 80% of Ca(2+) binding sites in the synaptic terminal of retinal bipolar cells were associated with mobile molecules that suppressed activation of Ca(2+)-sensitive K(+) channels with an efficiency equivalent to approximately 1.2 mM BAPTA. Removing these buffers caused a 30-fold increase in the number of vesicles released by Ca(2+) tail currents lasting approximately 0.5 ms and a 2-fold increase in the rapidly releasable pool of vesicles (RRP). The effects of BAPTA and EGTA indicate that vesicles comprising the RRP were docked at variable distances from Ca(2+) channels. We propose that endogenous Ca(2+) buffers regulate the size of the RRP by suppressing the release of vesicles toward the periphery of the active zone.
Translated title of the contributionEndogeonous calcium buffers regulate fast exocytosis in the synaptic terminal of retinal biploar cells
Original languageEnglish
Pages (from-to)101 - 112
Number of pages10
JournalNeuron
Volume33(1)
Publication statusPublished - Jan 2002

Bibliographical note

Publisher: Elsevier

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