Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning

James P.K. Armstrong, Jennifer L. Puetzer, Andrea Serio, Anne Géraldine Guex, Michaella Kapnisi, Alexandre Breant, Yifan Zong, Valentine Assal, Stacey C. Skaalure, Oisín King, Tara Murty, Christoph Meinert, Amanda C. Franklin, Philip G. Bassindale, Madeleine K. Nichols, Cesare M. Terracciano, Dietmar W. Hutmacher, Bruce W. Drinkwater, Travis J. Klein, Adam W. PerrimanMolly M. Stevens*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)

25 Citations (Scopus)
154 Downloads (Pure)

Abstract

Tissue engineering has offered unique opportunities for disease modelling and regenerative medicine, however, the success of these strategies is dependent upon faithful reproduction of native cellular organization. Here, we report that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibited significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic pattering of myoblasts in gelatin methacryloyl hydrogels significantly enhanced myofibrillogenesis and promoted the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120-150 μm and a spacing of 180-220 μm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically-patterned cells. We anticipate that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially-organized cell cultures, organoid development and bioelectronics.
Original languageEnglish
Article number1802649
Number of pages7
JournalAdvanced Materials
Volume30
Issue number43
Early online date12 Sep 2018
DOIs
Publication statusPublished - 25 Oct 2018

Keywords

  • acoustic
  • muscle
  • patterning
  • tissue engineering
  • ultrasound standing waves

Fingerprint Dive into the research topics of 'Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning'. Together they form a unique fingerprint.

  • Projects

    Functional Biomolecular Liquids

    Perriman, A. W.

    1/10/1330/09/18

    Project: Research

    Cite this

    Armstrong, J. P. K., Puetzer, J. L., Serio, A., Guex, A. G., Kapnisi, M., Breant, A., Zong, Y., Assal, V., Skaalure, S. C., King, O., Murty, T., Meinert, C., Franklin, A. C., Bassindale, P. G., Nichols, M. K., Terracciano, C. M., Hutmacher, D. W., Drinkwater, B. W., Klein, T. J., ... Stevens, M. M. (2018). Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning. Advanced Materials, 30(43), [1802649]. https://doi.org/10.1002/adma.201802649