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Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis

Research output: Contribution to journalArticle

  • Miller Huang
  • Jignesh Tailor
  • Qiqi Zhen
  • Aaron H. Gillmor
  • Matthew L. Miller
  • Holger Weishaupt
  • Justin Chen
  • Tina Zheng
  • Emily K. Nash
  • Lauren K. McHenry
  • Zhenyi An
  • Fubaiyang Ye
  • Yasuhiro Takashima
  • James Clarke
  • Harold Ayetey
  • Florence M.G. Cavalli
  • Betty Luu
  • Branden S. Moriarity
  • Shirin Ilkhanizadeh
  • Lukas Chavez
  • Chunying Yu
  • Kathreena M. Kurian
  • Thierry Magnaldo
  • Nicolas Sevenet
  • Philipp Koch
  • Steven M. Pollard
  • Peter Dirks
  • Michael P. Snyder
  • David A. Largaespada
  • Yoon Jae Cho
  • Joanna J. Phillips
  • Fredrik J. Swartling
  • A. Sorana Morrissy
  • Marcel Kool
  • Stefan M. Pfister
  • Michael D. Taylor
  • Austin Smith
  • William A. Weiss
Original languageEnglish
Pages (from-to)433-446.e7
Number of pages22
JournalCell Stem Cell
Volume25
Issue number3
Early online date13 Jun 2019
DOIs
DateAccepted/In press - 13 May 2019
DateE-pub ahead of print - 13 Jun 2019
DatePublished (current) - 5 Sep 2019

Abstract

Human neural stem cell cultures provide progenitor cells that are potential cells of origin for brain cancers. However, the extent to which genetic predisposition to tumor formation can be faithfully captured in stem cell lines is uncertain. Here, we evaluated neuroepithelial stem (NES) cells, representative of cerebellar progenitors. We transduced NES cells with MYCN, observing medulloblastoma upon orthotopic implantation in mice. Significantly, transcriptomes and patterns of DNA methylation from xenograft tumors were globally more representative of human medulloblastoma compared to a MYCN-driven genetically engineered mouse model. Orthotopic transplantation of NES cells generated from Gorlin syndrome patients, who are predisposed to medulloblastoma due to germline-mutated PTCH1, also generated medulloblastoma. We engineered candidate cooperating mutations in Gorlin NES cells, with mutation of DDX3X or loss of GSE1 both accelerating tumorigenesis. These findings demonstrate that human NES cells provide a potent experimental resource for dissecting genetic causation in medulloblastoma.

    Research areas

  • human pluripotent stem cells, medulloblastoma, neuroepithelial stem cells, SHH

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Elsevier at https://www.sciencedirect.com/science/article/pii/S1934590919302176?via%3Dihub#!. Please refer to any applicable terms of use of the publisher.

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    Licence: CC BY

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